Agrisera IncuBlocker (rabbit antibody)
Kit components: Wash Buffer (Reagent A) and the "all in one" goat anti-rabbit HRP-conjugated secondary antibody in advanced blocking solution (Reagent B).Required: primary antibody of your choice and
AgriseraECL SuperBright
This reagent offers extreme low femto level sensitivity combined with low background and superior signal to noise ratios.
Donkey normal serum (10 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot