SuperScript II Reverse Transcriptase (RT) is an improved version of SuperScript RT.

It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA,

 DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point

 mutations in the RNase H active center to reduce RNase H activity. This structural modification

 eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript

 II RT superior performance characteristics.

Yield – Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA – Generation of RT-PCR products up to 12 kb
Thermostability – Full activity at 42°C
Flexibility – Uses either total or poly(A)⁺ RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease,

 and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of SuperScript II is the amount