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For research use only. Not for use in diagnostic procedures.

ABI’s Standard PCR Controls can be used as positive controls in nucleic acid amplification reactions. ABI supplies virus infected cell DNA controls, and virus transformed cell DNA controls, which can be used as positive controls in standard PCR.

ABI’s Standard PCR Controls are purified from one of the following sources:

• Infected Cells: transduced or pathogen-infected cell cultures
• Transformed Cells: cells transformed by specific viruses
• Human Plasma

Virus infected cell DNA controls are isolated from virus infected cells and contain cellular and viral DNA in unknown proportions. Virus transformed cell DNA controls are isolated from cells transformed by human papillomavirus (HPV) type 16 or type 18 and contain HPV viral DNA integrated into the cell genome. Both virus infected and virus transformed DNAs are supplied at a DNA concentration (viral plus cellular DNA) of 50 ng/µL. Standard PCR Controls are purified from appropriate starting material, and the concentration is determined by fluorometry. Custom nucleic acid preparations are also available.

Details（注意事项）:

Shipping and Storage: This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Recommendations: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.CAUTION: ABI does not recommend storage of dilutions of quantitative DNA Controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to “lose” copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.

Safe Handling Recommendation: The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

Shipping and Storage: This product is shipped frozen on dry ice. Store at 70˚C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Recommendations: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.

Safe Handling Recommendation: The RNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product in not considered biohazardous. However, this lot was not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

 FAO（常见问题）：

How are Standard PCR controls supplied?  Standard PCR controls are supplied at a DNA concentration (viral plus cellular DNA) of 50 ng/µl. The concentration is determined by fluorometry.

Can a copy number be determined for Standard PCR Controls?   No, viral infected cell DNA controls and viral transformed DNA controls are isolated from  virus infected cells or virus transformed cells and therefore, these controls contain both cellular  and viral DNA in unknown and variable proportions.

How is the concentration of virus infected cell DNA controls and virus transformed  cell DNA controls determined?   The concentration of these DNAs is determined using fluorometry. These DNAs are intended primarily as positive controls in PCR reactions.

Why is the DNA concentration determined by fluorometry rather than the more  common absorbance method?  Fluorometric determinations virtually eliminate any skewing of the absorbance readings by  interfering contaminants such as RNA, single-stranded DNA, oligonucleotides or nucleotides.

Applications for use（适用范围）: 

• PCR
• Nucleic Acid-Based Assay

Quality control testing includes（质量控制检测包括）:

• PCR