HISTRAP FF CRUDE
产品名称： HISTRAP FF CRUDE
英文名称： HISTRAP FF CRUDE
产品编号： 17-5286-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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- 邮编 : 201900
- 所在区域 : 上海
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- 邮箱 : 342786402@qq.com
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HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates
- Optimized for purification of histidine-tagged* proteins directly from homogenized, unclarified cell lysates.
- Simply homogenize and load your sample directly.
- High binding capacity, approx. 40 mg/ml medium and negligible Ni2+ leakage.
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
- Convenient kit provides columns and buffers for quick and easy purifications.
- Simple operation with a syringe, pump, or chromatographic system such as ÄKTAdesign
HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates
Technical Information
* For licensing information, see Legal Info.
Customer feedback on Ni Sepharose 6 Fast Flow.
| TECHNICAL SPECIFICATIONS | |
| Medium | Ni Sepharose 6 Fast Flow |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1 ml) 1.6 × 2.5 cm (5 ml) |
| Dynamic binding capacity* | Approx. 40-mg histidine-tagged protein/ml medium |
| Recommended flow rate† | 1 ml/min (1 ml) 5 ml/min (5 ml) |
| Max. pressure† | 3 bar (0.3 MPa, 42 psi) |
| Compatibility during use | Stable in all commonly used buffers, reducing agents, denaturing agents and detergents, see Ni Sepharose™ High Performance - High-performance Purification |
| Chemical stability‡ | For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS |
| pH stability‡ | 2-14 (short term), 3-12 (long term) |
| Storage | 20% ethanol |
| Storage temperature | +4°C to 30°C |
| * Protein-dependent † H2O at room temperature ‡ Ni2+-stripped medium
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HisTrap™ FF crude columns allow simple, one-step purification of histidine-tagged proteins without sample pretreatment such as centrifugation and filtration.
HisTrap™ FF crude 1-ml and 5-ml columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates. Direct loading of unclarified sample both saves time and prevents degradation of sensitive target protein.
HisTrap™ FF crude columns are prepacked with Ni Sepharose™ 6 Fast Flow. For more information see Ni Sepharose™ 6 Fast Flow - Scale-up Purification. For column technical specifications, see HisTrap™ FF Columns - Easy Scale-up Purification (HisTrap™ FF Columns). For best results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, e.g. by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.
HisTrap FF crude Kit provides convenient purification of histidine-tagged proteins. The kit contains three HisTrap FF crude 1-ml columns, phosphate buffer, pH 7.4 (2 × 50 ml [8× concentrate]), 2 M imidazole, pH 7.4, all necessary connectors, a syringe, and instructions.
<sub>6</sub> from <italic>E. coli</italic> extract on HisTrap FF crude 1<UNspace/>ml. The medium was subjected to Ni<sup>2+</sup>-ion stripping, cleaning-in-place, and recharging between runs.'))
Four repeated purifications of MBP-(His)6 from E. coli extract on HisTrap FF crude 1 ml. The medium was subjected to Ni2+-ion stripping, cleaning-in-place, and recharging between runs.
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HisTrap FF crude columns save time in the purification of histidine-tagged proteins compared to conventional IMAC.
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