pcDNA.3.1-v5-his_B
产品名称： pcDNA.3.1-v5-his_B
英文名称： pcDNA.3.1-v5-his_B 质粒图谱序列抗性说明书价格报价
产品编号： BiovectorpcDNA.3.1-B
产品价格： 0
产品产地： Biovector Inc. USA
品牌商标： Biovector, Addgene, ATCC, Invi
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
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- 邮箱 : Biovector@163.com
Product Information
|
Order ID |
Name |
Description |
|
BiovectorpcDNA.3.1-v5-his_B |
pcDNA.3.1-v5-his_B |
pcDNA.3.1-v5-his_B. in E.coli, AmpR,TetR. Storage:4℃ |
载体信息：
|
Plasmid Type: |
Mammalian Expression |
|
Promotor: |
CMV |
|
Expression Level: |
High |
|
Cloning Method: |
Restriction Enzyme |
|
Size: |
5506 |
|
5 Sequencing 1 Primer: |
CMV-F |
|
3 Sequencing 1 Primer: |
BGH-rev |
|
5 Terminal: |
C-Term |
|
5 Terminal 2: |
C-Term |
|
Tag 1: |
V5 |
|
Tag 2: |
Myc |
|
Bacterial Resistance: |
Ampicillin |
|
Selectable Marker: |
Neomycin |
|
Notes: |
Vector for expressing C-terminally V5- and 6xHis-tagged proteins in mammalian cells. |
|
Stable: |
Unspecified |
|
Constitutive: |
Unspecified |
|
Viral/Non-Viral: |
Nonviral |
载体序列：
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC 50
51 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT 100
101 GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG 150
151 GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG 200
201 CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC 250
251 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 300
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG 350
351 CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT 400
401 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 450
451 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC 500
501 CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 550
551 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 600
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 650
651 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA 700
701 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 750
751 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG 800
801 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG 850
851 GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT 900
901 TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCTG 950
951 CAGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGCGGTTC 1000
1001 GAAGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGCGTAC 1050
1051 CGGTCATCAT CACCATCACC ATTGAGTTTA AACCCGCTGA TCAGCCTCGA 1100
1101 CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 1150
1151 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 1200
1201 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG 1250
1251 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT 1300
1301 GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG 1350
1351 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 1400
1401 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 1450
1451 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 1500
1501 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 1550
1551 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 1600
1601 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 1650
1651 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 1700
1701 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 1750
1751 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 1800
1801 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 1850
1851 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 1900
1901 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 1950
1951 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 2000
2001 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 2050
2051 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 2100
2101 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC 2150
2151 GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 2200
2201 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 2250
2251 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 2300
2301 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 2350
2351 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 2400
2401 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT 2450
2451 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 2500
2501 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 2550
2551 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 2600
2601 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 2650
2651 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 2700
2701 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG 2750
2751 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 2800
2801 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 2850
2851 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA 2900
2901 GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 2950
2951 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTT