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Layilin Antibody Datasheet
 
 
 
Layilin抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human Layilin protein ( Catalog#10208-H08H )
 
 
 
 
 
Clone ID :
 
 
 
1G1G4B12
 
 
 
 
抗体宿主 :
 
 
Mouse  IgG1
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human Layilin (rhLayilin; Catalog#10208-H08H; aa 1 - 220; NP_849156.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
Layilin抗体使用指南
 
 
 
 
特异性 : 
 
 
Human  Layilin
 
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
 
Western blot : 
 
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect layilin in WB. Using a DAB detection system, the detection limit for Layilin is approximately 20ng/lane under reducing conditions. Use of this antibody under non-reducing conditions is not recommended.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
 Avoid repeated freeze-thaw cycles.
 
 
 
 
 Layilin Antibody Related Products & Topics
 
 
相关研究领域:
 
Cancer>>Angiogenesis>>ECM Molecules in Angiogenesis>>Layilin
 
Immunology>>Adhesion Molecule>>Extracellular Matrix Molecule>>Hyaluronic Acid Binding Protein>>Layilin
 
Immunology>>Adhesion Molecule>>Lectin>>Layilin
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Layilin
 
Human
 
Layilin/Fc Protein, Recombinant 
 
10208-H02H
 
 
 
Layilin
 
Human
 
Layilin Protein, Recombinant
 
10208-H08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human 
 Layilin
 
WB
 
Mouse Monoclonal Antibody
 
10208-MM01
 
 
 
Human 
 Layilin
 
ELISA
 
Mouse Monoclonal Antibody
 
10208-MM02
 
 
 
Human 
 Layilin
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
10208-RP01
 
 
 
 
 
 Layilin Antibody Background
 
 
Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. In addition, merlin and radixin have been identified as different intracellular binding partners of Layilin. Accordingly, it has been suggested that Layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction.    
 
 
参考文献
 
 
1.  Rees, D.J.G. et al., 1990, Nature. 347: 685-689.
 
2.  Borowsky, M.L. et al., 1998, J. Cell Biol. 143: 429-442.
 
3.  Schoenwaelder, S.M. et al., 1999, Curr Opin Cell Biol. 11:274-286.
 
4.  Bono, P. et al., 2001, Mol. Biol. Cell. 12: 891-900.
 
5.  Bono, P. et al., 2005, Exp.Cell. Res. 308: 177-187.
 
 
 
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