HITRAP IMAC HP
产品名称： HITRAP IMAC HP
英文名称： HITRAP IMAC HP
产品编号： 17-0920-03
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
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IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
- For optimizing purification of histidine-tagged* proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Small bead size for high performance purification.
- High binding capacity.
- Prepacked HiTrap columns for convenient purification.
IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
Technical Information
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
* For licensing information, see Legal Info.
| TECHNICAL SPECIFICATIONS | |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Binding capacity‡ | At least 40 mg histidine-tagged protein/ml medium when charged with Ni2+ |
| Compatibility | Stable in all commonly used buffers |
| Chemical stability* | For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1M NaOH, 70% acetic acid 30 min tested 30% 2-propanol 1 h tested 2% SDS |
| pH stability* | 3-12 (long term), 2-14 (short term) |
| HiTrap™ prepacked columns | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate† | 1 ml/min (1 ml), 5 ml/min (5 ml) |
| Max. pressure† | 3 bar (0.3 MPa, 42 psi) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Medium uncharged with metal ions. † H2O at room temperature. ‡ Protein- and metal-ion dependent.
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<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
Purification of GFP-(His)6 from E. coli lysate using HiTrap IMAC HP column charged with different metal ions.
<sub>6</sub> fractions purified with different metal ions according to the chromatogram above. '))
SDS-PAGE of GFP-(His)6 fractions purified with different metal ions according to the chromatogram above
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=166316