BitNuclease.

Introduction

BiotoolTM BitNuclease is a universal endonuclease that is genetically engineered from Serratia marcescens extracellular endonuclease. It digests both single and doubled stranded RNA and DNA (in either linear, circular or super coiled form) into 3-5 base fragments, which is below the hybridization limit. BitNuclease has high activity and broad substrate tolerance, making it an ideal enzyme for removal of nucleic acids during protein preparation. It can be added along with cell lysis buffer to eliminate viscosity and increase yields of proteins, including nuclear proteins.
Also Known As: Benzonase®, Universal Nuclease, Extracellular Endonuclease from Serratia Marcescens

Highlights

Viscosity Reduction

Easy to pipette and filter. Accurate sample loading. Clear PAGE bands. Higher protein yields.

Nucleic Acid Removal.

Eliminate hydrophobic-binding of nucleic acids on chromatography columns. Industrial use in purification of vaccines, antibodies and other biologicals. Pharmaceutical use in the removal of DNA contamination.

Cost Effective

Competitive pricing makes BitNuclease affordable for everyone in protein research.

Applications

Complete, nonspecific digestion of all forms of DNA and RNA.
Purification of proteins, vaccines, antibodies and other biologicals.
Reduction of viscosity caused by nucleic acids.
Sample preparation for protein electrophoresis and chromatography.

Suggested Usage

Use for protein expression and production in an E. coli culture system

-30 minutes processing at room temperature: For every 1 gram of bacteria, add 250U BitNuclease, resuspended in cell lysis buffer or protein purification buffer. 
-30 minutes processing at 4oC: For every 1 gram of bacteria, add 1000U BitNuclease, resuspended in cell lysis buffer or protein purification buffer.

Cell culture protein sample preparation

First, dilute BitNuclease 1:10 with dilution buffer to 25U/µL. For every 100µL cell lysate, add 0.5-1µL 25U/µL BitNuclease.

Note: BitNuclease requires 1–2 mM Mg2+ for activation, please make sure Mg2+ is added in the solution.

Product Specification

BitNuclease 250U/µL 20µL
Dilution buffer 200µL

Dilution buffer formula: 50mM Tris-HCl, pH 8.0, 20mM NaCl, 5mM MgCl2, 50% Glycerol.

Unit definition

One enzymatic unit will digest salmon sperm DNA to a ΔA260 of 1.0 (corresponding to degradation of 37µg DNA) in 30 min at pH 8.0 at 37 °C

Working scope

BitNuclease is functional between pH 6 and 10, with best efficiency in pH 8-8.5. Functional temperature is from 0 °C to 44 °C and optimal temperature is between 35 °C-42 °C.
BitNuclease can tolerate buffers containing up to 150mM monovalent cation , 100mM phosphate, 100mM ammonium sulfate, 100mM Guanidine HCl, 0.1% SDS, 1% Triton X-100, 1% Tween 20. This is compatible with most buffers used in protein purification.

Storage

This product can be stored at -20 °C for 2 years, or 4 °C for 2 months.

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