来源
用途
交叉反应性
分子量
Rabbit
WB, IP, IF, IHC, ICC, FC
H, M, R
21.0kDa
       WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
       H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
       配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
       建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000-1:2,000
1:10-1:50
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
-
-
抗体详细信息如下:
About this Antibody
Name
DIABLO Rabbit Monoclonal Antibody
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
Affinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
56616(Human); 66593(Mouse)
SwissProt
Q9NR28(Human); Q9JIQ3(Mouse)
Synonyms
DFNA64; SMAC
Category
Apoptosis
Background
The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.
包装清单:
产品编号
产品名称
包装
AF2092
DIABLO Rabbit Monoclonal Antibody
50μl
AZ050
Western一抗稀释液
50ml
-
说明书
1份
保存条件:
       DIABLO Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
       如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
       回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
       提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。
代表性图片：
 
Fig. 1. Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat; Lane 2: Hela; Lane 3: MCF-7
 
Fig. 2. ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
 
Fig. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
 
Fig. 4. Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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