HiTrap Capto DEAE-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
HiTrap Capto DEAE

HiTrap Capto DEAE

商家询价

产品名称： HiTrap Capto DEAE

英文名称： HiTrap Capto DEAE

产品编号： 28-9165-37

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
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28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

Capto IEX Media/HiTrap Capto IEX Columns

  • High-throughput medium for capture and intermediate purification at process-scale.
  • Combined high binding capacity, high flow rate, and low backpressure properties reduce process cycle times and improve productivity.
  • Rigid agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
  • Flow rates of up to 700 cm/h can be employed at process-scale with a backpressure below 3 bar.
  • HiTrap™ prepacked column format for easy screening and convenient process development.

Capto IEX Media/HiTrap Capto IEX Columns

Technical Information


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Capto™ Q has a strong quaternary ammonium (Q) anion exchanger, Capto DEAE has a weak diethylaminoethyl anion exchanger, and Capto S has a strong sulfoethyl cation exchanger coupled to a chemically modified, high-flow agarose matrix. The modified agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix.

 

Capto™ ViralQ* is in all respects identical to Capto Q, except that a license for use of the product for virus purification is included.

 

Prepacked HiTrap™ Capto™ Q, HiTrap Capto DEAE (strong and weak anion exchange, respectively), and HiTrap™ Capto™ S (strong cation exchange) columns are ideal for small scale purifications. HiScreen columns prepacked with the same media are excellent tools for method optimization in process development, such as screening of selectivity, binding and elution conditions.

 

* See licensing information at back of catalog.

 

Capto ion exchangers increase productivity and shorten overall process time
Stationary phase Process cycle time (min) Productivity (kg/m3/h)
Capto™ DEAE‡ 197 37
DEAE Sepharose Fast Flow‡ 306 12
Capto™ Q* 91 11
Q Sepharose™ Fast Flow* 190 3
Capto S† 131 53
SP Sepharose™ Fast Flow† 229 17
* Productivity calculations are based on purification of GFP in E. coli homogenate.
† Productivity calculations are based on purification of a-chymotrypsin in E. coli homogenate.
‡ Productivity calculations are based on pure amyloglucosidase.

 

 

TECHNICAL SPECIFICATIONS
Ion exchanger type   
Capto Q  Quaternary ammonium, strong anion 
Capto DEAE  Diethylaminoethyl, weak anion 
Ionic capacity   
Capto Q  0.16-0.22 mmol Cl-/ml 
Capto S  0.11-0.14 mmol Na+/ml 
Capto DEAE  0.29¾0.35 mmol Cl-/ml 
Average particle size  90 μm 
Matrix  Highly cross-linked agarose with dextran surface extender 
Dynamic binding capacity 
Capto Q  Min. 100 mg BSA/ml medium capacity at  10% breakthrough, 1-min residence time 
Capto S  Min. 120 mg lysozyme/ml medium capacity  at 10% breakthrough, 1-min residence time 
Capto DEAE  Min. 90 mg ovalbumin/ml medium at 10% breakthrough, 1-min residence time 
Pressure/flow spec.  3 bar at 700 cm/h, 1-m diameter column,  20-cm bed height in water 
pH stability   
Capto Q  2-14 (short term), 2-12 (long term) 
Capto S  3-14 (short term), 4-12 (long term) 
Capto DEAE  2-14 (short term), 2-12 (long term) 
Chemical stability   Stable in all commonly used aqueous buffers: 8 M urea, 6 M Gua-HCl, 70% ethanol, 1 M NaOH and 1 M acetic acid. 
Storage  20% ethanol (Q, DEAE), 0.2 M sodium acetate in 20% ethanol (S) 
Storage temperature  4°C to 30°C 
HiTrap prepacked columns
Column volume  1 ml and 5 ml 
Column dimensions*  0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml) 
Max. pressure†  3 bar (0.3 MPa) 
* Given the short bed height of the HiTrap™ column, the flow rate should not exceed 150 cm/h to achieve 1-min residence time for high dynamic binding capacity.
† H2O at 25°C

 

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