单位定义

One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

反应条件

1X Isothermal Amplification Buffer Pack
Incubate at 65°C

1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
50 mM KCl
2 mM MgSO₄
0.1% Tween® 20
(pH 8.8 @ 25°C)

贮存溶液

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.1 @ 25°C

热失活

80°C for 20 minutes

单位活性检测条件

50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl₂, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [³H]-dTTP and 100 μg/ml BSA.

应用特性

• Isothermal amplification (LAMP)
• Applications requiring strand-displacement DNA synthesis
• DNA sequencing through high GC regions
• Rapid sequencing from nanogram amounts of DNA template