pCDH-EF1-MCS-T2A-Puro
产品名称： pCDH-EF1-MCS-T2A-Puro
英文名称： pCDH-EF1-MCS-T2A-Puro
产品编号： Biovector-01128569
产品价格： 0
产品产地： Biovector Inc. USA
品牌商标： Biovector, Addgene, ATCC, Invi
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Product Data Sheet
|
Order ID |
Name |
Description |
|
Biovector-01128569 |
pCDH-EF1-MCS-T2A-Puro |
pCDH-EF1-MCS-T2A-Puro,DNA, 15uL,AmpR.Storage:-20℃ |
Transformation of plasmid DNA to competent E. Coli cells
- Thaw competent cells on ice. 20–200µL per tube
- Add 3µL of plasmid
- Mix very gently!
- Incubate the tubes on ice for 30 min
- Heat shock the cells for 45 sec to 2 min at 42°C
- Place the tubes immediately on ice for at least 2 min
- Add 800µL of SOC medium to each tube
- Incubate for 1 hour at 37°C and shake vigorously
- Spin down briefly and remove most supernatants
- Resuspend cell pellet with the rest SOC medium in the tube by pipetting
- Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C
载体信息：
|
质粒类型: |
慢病毒载体 |
|
克隆方法: |
多克隆位点，限制性内切酶 |
|
载体大小: |
7095 bp |
|
载体抗性: |
Ampicillin (氨苄青霉素) |
载体序列：
LOCUS CD527A_1_pCDH_EF 7094 bp ds-DNA linear 30-JAN-2013
DEFINITION .
ACCESSION
VERSION
SOURCE .
ORGANISM .
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_feature 570..923
/label=Gag
/ApEinfo_fwdcolor=#d70428
/ApEinfo_revcolor=#d70428
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 235..414
/label=HIV/LTR
/ApEinfo_fwdcolor=#ff8040
/ApEinfo_revcolor=#ff8040
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1079..1311
/label=RRE
/ApEinfo_fwdcolor=#ff80ff
/ApEinfo_revcolor=#ff80ff
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1..234
/label=RSV Promoter
/ApEinfo_fwdcolor=#800080
/ApEinfo_revcolor=#690f96
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1312..1807
/label=env
/ApEinfo_fwdcolor=#0080c0
/ApEinfo_revcolor=#0080c0
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1808..1925
/label=cPPT
/ApEinfo_fwdcolor=#008000
/ApEinfo_revcolor=#008000
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1937..2480
/label=EF1
/ApEinfo_fwdcolor=#ebabe0
/ApEinfo_revcolor=#d991e3
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 2517..2570
/label=T2A/peptide
/ApEinfo_fwdcolor=#00ffff
/ApEinfo_revcolor=#00ffff
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 2577..3173
/label=PuroR
/ApEinfo_fwdcolor=#db7e6f
/ApEinfo_revcolor=#d6804b
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 3189..3779
/label=WPRE
/ApEinfo_fwdcolor=#86dd8a
/ApEinfo_revcolor=#b0ffb0
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 3851..4084
/label=3LTR
/ApEinfo_fwdcolor=#0080ff
/ApEinfo_revcolor=#0080ff
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 4156..4442
/label=SV40/EEL/poly(A)s
/ApEinfo_fwdcolor=#8080ff
/ApEinfo_revcolor=#8080ff
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 4812..5485
&n