pAdenoX-PRLS-ZsGreen1载体质粒
产品名称： pAdenoX-PRLS-ZsGreen1载体质粒
英文名称： pAdenoX-PRLS-ZsGreen1载体质粒
产品编号： BiovectorpAXPDSZ
产品价格： 0
产品产地： Biovector Inc. USA
品牌商标： Biovector, Addgene, ATCC, Invi
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Product Data Sheet
|
Order ID |
Name |
Description |
|
BiovectorpAXPDSZ |
pAdenoX-PRLS-ZsGreen1 |
pAdenoX-PRLS- ZsGreen1, 15uL DNA. AmpR.Storage:-20℃ |
Standard heat-shock transformation of chemically competent bacteria
- Take competent cells out of -80°C and thaw on ice.
- Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
- Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
- Place the competent cell/DNA mixture on ice for 20-30min.
- Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
- Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.
- Plate all of the transformation onto a 10cm LB agar plate containing the appropriate antibiotic.
- Incubate plates at 37°C overnight.
Location of Features
• Screening Primer (reverse) [complementary]: 19–38
• PCMV IE (human cytomegalovirus immediate early promoter): 24436–25024
• ZsGreen1: 25049–25741
• SV40 polyA signals: 25897–25947
• ITR (inverted terminal repeat): 30908–30967
• Ampr (ampicillin resistance gene; β-lactamase): 31868–32728
• pUC origin of replication: 33373–33546
• ITR (inverted terminal repeat): 33957–34016
• Screening Primer (forward): 34060–34084
• Ψ (packaging signal): 34149–34297
1 GGAGAAAGAG GTAATGAAAT GGCATCGACT CGAAGATCTG GGCGTGGTTA 50
51 AGGGTGGGAA AGAATATATA AGGTGGGGGT CTTATGTAGT TTTGTATCTG 100
101 TTTTGCAGCA GCCGCCGCCG CCATGAGCAC CAACTCGTTT GATGGAAGCA 150
151 TTGTGAGCTC ATATTTGACA ACGCGCATGC CCCCATGGGC CGGGGTGCGT 200
201 CAGAATGTGA TGGGCTCCAG CATTGATGGT CGCCCCGTCC TGCCCGCAAA 250
251 CTCTACTACC TTGACCTACG AGACCGTGTC TGGAACGCCG TTGGAGACTG 300
301 CAGCCTCCGC CGCCGCTTCA GCCGCTGCAG CCACCGCCCG CGGGATTGTG 350
351 ACTGACTTTG CTTTCCTGAG CCCGCTTGCA AGCAGTGCAG CTTCCCGTTC 400
401 ATCCGCCCGC GATGACAAGT TGACGGCTCT TTTGGCACAA TTGGATTCTT 450
451 TGACCCGGGA ACTTAATGTC GTTTCTCAGC AGCTGTTGGA TCTGCGCCAG 500
501 CAGGTTTCTG CCCTGAAGGC TTCCTCCCCT CCCAATGCGG TTTAAAACAT 550
551 AAATAAAAAA CCAGACTCTG TTTGGATTTG GATCAAGCAA GTGTCTTGCT 600
601 GTCTTTATTT AGGGGTTTTG CGCGCGCGGT AGGCCCGGGA CCAGCGGTCT 650
651 CGGTCGTTGA GGGTCCTGTG TATTTTTTCC AGGACGTGGT AAAGGTGACT 700
701 CTGGATGTTC AGATACATGG GCATAAGCCC GTCTCTGGGG TGGAGGTAGC 750
751 ACCACTGCAG AGCTTCATGC TGCGGGGTGG TGTTGTAGAT GATCCAGTCG 800
801 TAGCAGGAGC GCTGGGCGTG GTGCCTAAAA ATGTCTTTCA GTAGCAAGCT 850
851 GATTGCCAGG GGCAGGCCCT TGGTGTAAGT GTTTACAAAG CGGTTAAGCT 900
901 GGGATGGGTG CATACGTGGG GATATGAGAT GCATCTTGGA CTGTATTTTT 950
951 AGGTTGGCTA TGTTCCCAGC CATATCCCTC CGGGGATTCA TGTTGTGCAG 1000
1001 AACCACCAGC ACAGTGTATC CGGTGCACTT GGGAAATTTG TCATGTAGCT 1050
1051 TAGAAGGAAA TGCGTGGAAG AACTTGGAGA CGCCCTTGTG ACCTCCAAGA 1100
1101 TTTTCCATGC ATTCGTCCAT AATGATGGCA ATGGGCCCAC GGGCGGCGGC 1150
1151 CTGGGCGAAG ATATTTCTGG GATCACTAAC GTCATAGTTG TGTTCCAGGA 1200
1201 TGAGATCGTC ATAGGCCATT TTTACAAAGC GCGGGCGGAG GGTGCCAGAC 1250
1251 TGCGGTATAA TGGTTCCATC CGGCCCAGGG GCGTAGTTAC CCTCACAGAT 1300
1301 TTGCATTTCC CACGCTTTGA GTTCAGATGG GGGGATCATG TCTACCTGCG 1350
1351 GGGCGATGAA GAAAACGGTT TCCGGGGTAG GGGAGATCAG CTGGGAAGAA 1400
1401 AGCAGGTTCC TGAGCAGCTG CGACTTACCG CAGCCGGTGG GCCCGTAAAT 1450
1451 CACACCTATT ACCGGCTGCA ACTGGTAGTT AAGAGAGCTG CAGCTGCCGT 1500
1501 CATCCCTGAG CAGGGGGGCC ACTTCGTTAA GCATGTCCCT GACTCGCATG 1550
1551 TTTTCCCTGA CCAAATCCGC CAGAAGGCGC TCGCCGCCCA GCGATAGCAG 1600
1601 TTCTTGCAAG GAAGCAAAGT TTTTCAACGG TTTGAGACCG TCCGCCGTAG 1650
1651 GCATGCTTTT GAGCGTTTGA CCAAGCAGTT CCAGGCGGTC CCACAGCTCG 1700
1701 GTCACCTGCT CTACGGCATC TCGATCCAGC ATATCTCCTC GTTTCGCGGG 1750
1751 TTGGGGCGGC TTTCGCTGTA CGGCAGTAGT CGGTGCTCGT CCAGACGGGC 1800
1801 CAGGGTCATG TCTTTCCACG GGCGCAGGGT CCTCGTCAGC GTAGTCTGGG 1850
1851 TCACGGTGAA GGGGTGCGCT CCGGGCTGCG CGCTGGCCAG GGTGCGCTTG 1900
1901 AGGCTGGTCC TGCTGGTGCT GAAGCGCTGC CGGTCTTCGC CCTGCGCGTC 1950
1951 GGCCAGGTAG CATTTGACCA TGGTGTCATA GTCCAGCCCC TCCGCGGCGT 2000
2001 GGCCCTTGGC GCGCAGCTTG CCCTTGGAGG AGGCGCCGCA CGAGGGGCAG 2050
2051 TGCAGACTTT TGAGGGCGTA GAGCTTGGGC GCGAGAAATA CCGATTCCGG 2100
2101 GGAGTAGGCA TCCGCGCCGC AGGCCCCGCA GACGGTCTCG CATTCCACGA 2150
2151 GCCAGGTGAG CTCTGGCCGT TCGGGGTCAA AAACCAGGTT TCCCCCATGC 2200
2201 TTTTTGATGC GTTTCTTACC TCTGGTTTCC ATGAGCCGGT GTCCACGCTC 2250
2251 GGTGACGAAA AGGCTGTCCG TGTCCCCGTA TACAGACTTG AGAGGCCTGT 2300
2301 CCTCGAGCGG TGTTCCGCGG TCCTCCTCGT ATAGAAACTC GGACCACTCT 2350
2351 GAGACAAAGG CTCGCGTCCA GGCCAGCACG AAGGAGGCTA AGTGGGAGGG 2400
2401 GTAGCGGTCG TTGTCCACTA GGGGGTCCAC TCGCTCCAGG GTGTGAAGAC 2450
2451 ACATGTCGCC CTCTTCGGCA TCAAGGAAGG TGATTGGTTT GTAGGTGTAG 2500
2501 GCCACGTGAC CGGGTGTTCC TGAAGGGGGG CTATAAAAGGTUNEology 波长可调检测卡盒-->