Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Other Notes

This poly-L-lysine is relatively high density which provides good binding characteristics, but it is more viscous and difficult to handle. It may be degraded by some cell types. In that case a poly-D-lysine should be chosen.

有关聚氨基酸的其他技术信息，请访问 聚氨基酸常见问题解答资源。

General description

Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.

The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.