Aspartame Assay Kit
产品名称: Aspartame Assay Kit
英文名称: Aspartame Assay Kit
产品编号: K-ASPTM
产品价格: 0
产品产地: 爱尔兰
品牌商标: Megazyme
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 上海市徐汇区蒲汇塘路11号博大商务楼1307室
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 138****4547 点击查看
- 传真 : 点击查看
- 邮箱 : 3bio@163.com
是一种简单可靠的方法,用于饮料和食品中甜味剂的特定测量和分析。
UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials
Principle:
(pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH
(dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine
(glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate
(L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+
Kit size: * 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition: Novel methodAdvantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Measures aspartame and breakdown products (L-aspartate and aspartame acid)
- Very specific
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats