Aspartame Assay Kit-相关检验试剂-试剂-生物在线
上海新睿生物科技有限公司
Aspartame Assay Kit

Aspartame Assay Kit

商家询价

产品名称: Aspartame Assay Kit

英文名称: Aspartame Assay Kit

产品编号: K-ASPTM

产品价格: 0

产品产地: 爱尔兰

品牌商标: Megazyme

更新时间: null

使用范围: null

上海新睿生物科技有限公司
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  • 邮箱 : 3bio@163.com

是一种简单可靠的方法,用于饮料和食品中甜味剂的特定测量和分析。

适用于手动,自动分析仪和微孔板格式。
 

UV-method for the determination of Aspartame (and breakdown 
products) in foodstuffs, beverages and other materials

Principle:
                       (pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

                      (dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

                      (glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

                                 (L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                            * 50 assays (manual) / 500 (microplate)
                                          / 500 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:      Novel method

Advantages

 

  • Very cost effective 
  • All reagents stable for > 12 months after preparation 
  • Only enzymatic kit available 
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid) 
  • Very specific 
  • Very rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats