FASTA Graphics

LOCUS       FJ797515                6722 bp    DNA     circular SYN 08-OCT-2009

DEFINITION  Expression vector pBADSce, complete sequence.

ACCESSION   FJ797515

VERSION     FJ797515.1  GI:226344766

KEYWORDS    .

SOURCE      Expression vector pBADSce

  ORGANISM  Expression vector pBADSce

            other sequences; artificial sequences; vectors.

REFERENCE   1  (bases 1 to 6722)

  AUTHORS   Lopez,C.M., Rholl,D.A., Trunck,L.A. and Schweizer,H.P.

  TITLE     Versatile dual-technology system for markerless allele replacement

            in Burkholderia pseudomallei

  JOURNAL   Appl. Environ. Microbiol. 75 (20), 6496-6503 (2009)

   PUBMED   19700544

REFERENCE   2  (bases 1 to 6722)

  AUTHORS   Lopez,C.M. and Schweizer,H.P.

  TITLE     Homing Endonuclease-based System for Select Agent-compliant

            Markerless Gene Replacement in Burkholderia pseudomallei

  JOURNAL   Unpublished

REFERENCE   3  (bases 1 to 6722)

  AUTHORS   Lopez,C.M. and Schweizer,H.P.

  TITLE     Direct Submission

  JOURNAL   Submitted (02-MAR-2009) Microbiology, Immunology and Patholofy,

            Colorado State University, 3205 Rampart Rd., Fort Collins, CO

            80523, USA

FEATURES             Location/Qualifiers

     source          1..6722

                     /organism="Expression vector pBADSce"

                     /mol_type="other DNA"

                     /db_xref="taxon:629243"

                     /note="pBADSce"

     regulatory      14..439

                     /regulatory_class="terminator"

                     /note="Escherichia coli rrnB fragment containing

                     terminators T1 and T2"

     gene            complement(930..1304)

                     /gene="ble"

     CDS             complement(930..1304)

                     /gene="ble"

                     /note="zeocin resistance protein"

                     /codon_start=1

                     /transl_table=11

                     /product="Ble"

                     /protein_id="ACO48885.1"

                     /db_xref="GI:226344767"

                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD

                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW

                     GREFALRDPAGNCVHFVAEEQD"

     regulatory      complement(1305..1371)

                     /regulatory_class="promoter"

                     /note="pEM7"

     gene            1855..2688

                     /gene="rep-ts"

     CDS             1855..2688

                     /gene="rep-ts"

                     /note="TS replication protein"

                     /codon_start=1

                     /transl_table=11

                     /product="Rep-TS"

                     /protein_id="ACO48886.1"

                     /db_xref="GI:226344768"

                     /translation="MASPPMVYKSNALVEAAYCLSVQEQRIVLASISQVKRSEPVTDE

                     VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV

                     QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMGSTHAIRLYELLTQWD

                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK

                     VTHLLFSFGPKKPAKAVGKAPAKRKARKISDAEIAKQARPGETWEAARARLTQMPLDL

                     A"

     rep_origin      2915..3323

                     /note="M13 replication origin"

     rep_origin      3379..4076

                     /note="pBBR22 replication origin"

     gene            complement(4769..5647)

                     /gene="araC"

     CDS             complement(4769..5647)

                     /gene="araC"

                     /note="arabinose operon subunit"

                     /codon_start=1

                     /transl_table=11

                     /product="AraC"

                     /protein_id="ACO48887.1"

                     /db_xref="GI:226344769"

                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG

                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA

                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL

     &nb