HISTRAP HP

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产品名称： HISTRAP HP

英文名称： HISTRAP HP

产品编号： 17-5247-01

产品价格： 0

产品产地：美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司

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地址 : 上海市宝山区友谊路1506号东智广场1109室
邮编 : 201900
所在区域 : 上海
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产品详情

17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML
17-5319-01	HISTRAP FF 5 X 1 ML
17-5319-02	HISTRAP FF 100 X 1 ML
17-5441-22	HITRAP CAPTO S, 5X1 ML
17-5441-23	HITRAP CAPTO S, 5X5 ML
17-5468-51	HiTrap Capto SP ImpRes 5x1mL
17-5468-55	HiTrap Capto SP ImpRes 5x5mL
17-5470-51	HiTrap Capto Q ImpRes 5x1mL
17-5470-55	HiTrap Capto Q ImpRes 5x5mL
17-6002-33	HITRAP IEX SELECTION KIT
18-1142-75	REC PROTEIN PURIF HANDBOOK
28-4010-81	HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77	HISTRAP FF CRUDE KIT
28-4017-45	GSTRAP 4B, 5 X 1 ML
28-4017-46	GSTRAP 4B, 100 X 1 ML
28-4017-47	GSTRAP 4B, 1 X 5 ML
28-4017-48	GSTRAP 4B, 5 X 5 ML
28-4017-49	GSTRAP 4B, 100 X 5 ML
28-4058-44	HiTrap Capto Adhere 5x1ml
28-4058-46	5x5 ml Hitrap Capto adhere
28-4082-53	HITRAP MABSELECT, 5 X 1 ML
28-4082-55	HITRAP MABSELECT, 1 X 5 ML
28-4082-56	HITRAP MABSELECT, 5 X 5 ML
28-4082-58	HITRAP MABSELECT XTRA, 5X1ML
28-4082-60	HITRAP MABSELECT XTRA, 1X5ML
28-4082-61	HITRAP MABSELECT XTRA, 5X5ML
28-4110-01	HiTrap Butyl HP, 5x1ml
28-4110-05	HiTrap Butyl HP, 5 x 5ml
28-4110-07	HiTrap HIC Selection Kit, 7x1
28-9075-46	StrepTrap HP 5 x 1mL
28-9075-47	StrepTrap HP 1 x 5mL
28-9075-48	StrepTrap HP 5 x 5mL
28-9165-37	HiTrap Capto DEAE, 5X1ML
28-9165-40	HiTrap Capto DEAE, 5X5ML
28-9187-78	MBPTrap 5 x 1mL
28-9187-79	MBPTrap 1 x 5 mL
28-9187-80	MBPTrap 5 x 5mL
28-9343-88	HiTrap Capto IEX Selection Kit
28-9520-85	HiTrap Con A 4B 5 x 1 ml
28-9520-96	HiTrap Con A 4B 5 x 5 ml

HisTrap™ HP Columns - Easy, High-performance Purification

Optimized for high-performance purification of histidine-tagged* proteins.
Ni Sepharose High Performance prepacked in convenient HiTrap 1-ml and 5-ml columns.
Negligible Ni²⁺ leakage.
High binding capacity, at least 40 mg/ml medium.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Simple manual operation with a syringe, pump, or chromatography system such as ÄKTAdesign

HisTrap™ HP Columns - Easy, High-performance Purification

Technical Information

* For licensing information, see Legal Info.

HisTrap HP 1-ml and 5-ml columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)₄ or (histidine)₁₀. The shorter (histidine)₄ will bind more weakly and the longer (histidine)₁₀ will bind more strongly compared with (histidine)₆. This difference in binding strength can be used during purification; since (histidine)₁₀ binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be co-purified with the tagged target protein.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

For convenient scaling up of histidine-tagged protein purification, use 20-ml HisPrep™ FF 16/10 columns. Ni Sepharose™ 6 Fast Flow, the medium prepacked in HisTrap™ FF and HisPrep™ FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.

HiTrap™ IMAC HP, see IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

For information on the complete range of products for histidine-tagged protein purification, see Introduction to Tagged Protein Purification.

TECHNICAL SPECIFICATIONS
Medium	Ni Sepharose™ High Performance
Column volume	1 ml and 5 ml
Dynamic binding capacity*	At least 40 mg histidine-tagged protein/ml medium
Column dimensions	0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
Recommended flow rate	1 ml/min (1 ml); 5 ml/min (5 ml)
Max flow rate^†	4 ml/min (1 ml); 20 ml/min (5 ml)
Max. pressure^†	0.3 MPa, 3 bar
pH stability ^‡	2-14 (short term), 3-12 (long term)
Compatibility	Stable in all commonly used buffers, reducing agents, denaturants and detergents (see Ni Sepharose™ High Performance - High-performance Purification) for more information.
Chemical stability	For more information see Introduction to Tagged Protein Purification.
Storage	20% ethanol
Storage temperature	4°C to 30°C
* Protein binding capacity is protein-to protein dependent.^† H₂O at room temperature^‡ Ni²⁺-stripped medium

HisTrap™ HP 1-ml and 5-ml columns for convenient high-resolution purification of histidine-tagged proteins.

Purification of GFP-(histidine)₆ on HisTrap HP 1 ml. Due to the small bead size of
Ni Sepharose High Performance (34 μm) the eluted pool was only 2 ml, resulting in a concentrated eluted target protein.

SDS-PAGE of pooled fractions. Inclusion of 65 mM imidazole in the sample and during the wash provided the highest possible purity with no loss of target protein.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165701