FITC标记的泛素蛋白连接酶D4抗体
产品名称： FITC标记的泛素蛋白连接酶D4抗体
英文名称： Anti-UBE2D4/FITC
产品编号： HZ-8354R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-UBE2D4/FITC Conjugated antibody
FITC标记的泛素蛋白连接酶D4抗体
| 产品编号 | bs-8354R-FITC |
| 英文名称 | Anti-UBE2D4/FITC |
| 中文名称 | FITC标记的泛素蛋白连接酶D4抗体 |
| 别 名 | HBUCE 1; HBUCE 1; HBUCE1; UB2D4_HUMAN; UBE2D 4; UBE2D4; Ubiquitin carrier protein D4; Ubiquitin conjugating enzyme 1 isoform; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-protein ligase D4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UBE2D4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation. Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family. Database links: UniProtKB/Swiss-Prot: Q9Y2X8.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
泛素化是一个重要的机制，通过它，三类酶协同作用于短命或异常蛋白质以破坏。泛素化涉及的三类酶是泛素激活酶（E1S）、泛素结合酶（E2S）和泛素蛋白连接酶（E3S）。泛素化过程的第一步需要泛素-C末端的ATP依赖性激活和由E1酶组装多泛素链。然后，泛素链与E2酶结合，生成中间泛素-E2复合物。然后，E3酶催化泛素从E2转移到适当的蛋白质底物，从而针对该底物进行降解。广泛的酶促进了这种蛋白水解泛素途径，其中一种是UBE2E2（也称为UBCH8在人类中），其作为E2酶发挥作用，并催化泛素与ATP相关的共价连接到靶蛋白，从而在蛋白质降解中发挥重要作用。