pET-17b图谱序列抗性标签酶切位点
产品名称: pET-17b图谱序列抗性标签酶切位点
英文名称: pET-17b图谱序列抗性价格Biovector质粒库
产品编号: 39568038764
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector中国质粒载体菌株细胞
更新时间: null
使用范围: null
pET17b载体基本信息
出品公司: EMD Biosciences(Novagen) 别名: pET17b, pet 17b 质粒类型: 大肠杆菌蛋白表达 表达水平: 高 克隆方法: 多克隆位点,限制性内切酶 载体大小: 3306bp 5' 测序引物: T7 5' 测序引物序列: 5'-TAATACGACTCACTATAGGG-3' 载体标签: N-T7 载体抗性: Ampicillin 备注: Hosts: E.coli. Related vectors: pBR322.产品目录号: 69663-3 稳定性: 瞬时表达 Transient 组成型: 组成型 Constitutive 病毒/非病毒: 非病毒 pET17b载体质粒图谱和多克隆位点信息
Feature Name
Start
End
T7_terminator 131 3 T7_Terminal_primer 71 89 T7_leader 269 237 Xpress_fwd_primer 270 252 T7_transl_en_RBS 294 278 T7_promoter 349 331 ROP 858 1049 pGEX_3_primer 1065 1043 Ampicillin 3098 2238 AmpR_promoter 3168 3140
ORF
Start
End
ORF frame 2 3098 2238
Enzyme Name
CutXhoI 141 NotI 147 EcoRV 166 EcoRI 174 SpeI 199 BamHI 205 SacI 215 KpnI 221 HindIII 223 NheI 261 NdeI 268 XbaI 306 BglII 364 MscI 389 PvuII 1009 AatII 3233 pET17b载体简介
The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
pET17b载体序列
LOCUS pET-17b 3306 bp DNA circular SYN
DEFINITION pET-17b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3306
/organism="pET-17b"
/mol_type="other DNA"
terminator complement(3..131)
/label="T7_terminator"
misc_feature 71..89
/label="T7_Terminal_primer"
misc_feature comp