HITRAP IEX SELECTION KIT
产品名称: HITRAP IEX SELECTION KIT
英文名称: HITRAP IEX SELECTION KIT
产品编号: 17-6002-33
产品价格: 0
产品产地: 美国
品牌商标: GE
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
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HiTrap™ IEX Selection Kit
- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1-ml HiTrap™ columns.
- Excellent for optimization studies: buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Excellent for screening different media: includes seven 1-ml HiTrap™ columns prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), ANX Sepharose™ 4 Fast Flow (high sub) (weak anion), Q Sepharose™ XL (strong anion), and SP Sepharose™ XL (strong cation), connectors, and instructions.
- Designed for use with syringe, peristaltic pump and other chromatography systems such as ÄKTA™design.
HiTrap™ IEX Selection Kit
Technical Information
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.
ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.
The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
TECHNICAL SPECIFICATIONS | |
Media | Q Sepharose™ Fast Flow |
SP Sepharose™ Fast Flow | |
CM Sepharose™ Fast Flow | |
DEAE Sepharose™ Fast Flow | |
ANX Sepharose™ 4 Fast Flow (high sub) | |
Q Sepharose™ XL | |
SP Sepharose™ XL | |
Column volume | 1 ml |
Max. flow rate* | 4 ml/min |
Recommended flow rate | 1 ml/min |
Max. back pressure | 3 bar (0.3 MPa, 43 psi) |
Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
Storage temperature | 4°C to 30°C |
* H2O at 25°C
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