英文名称Caspase 8
中文名称半胱氨酸蛋白酶8单克隆抗体
别    名Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5.  
研究领域细胞生物  神经生物学  细胞凋亡  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号4G12
交叉反应Human, Mouse,  (predicted: Rat, )
产品应用WB=1:2000-5000 ELISA=1:500-1000 IHC-P=1:400-1000 IHC-F=1:500-1000 ICC=1:100-500 IF=1:500-1000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43/57kDa
细胞定位细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原Recombinant human Caspase 8 Protein : 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
 
Function:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
 
Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2.
 
Subcellular Location:
Cytoplasm.
 
Tissue Specificity:
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
 
DISEASE:
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
 
Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
 
SWISS:
Q14790
 
Gene ID:
841
 
Database links:
Entrez Gene: 841 Human
 
Entrez Gene: 12370 Mouse
 
Entrez Gene: 54474 Rat
 
Entrez Gene: 64044 Rat
 
Omim: 601763 Human
 
SwissProt: Q14790 Human
 
SwissProt: O89110 Mouse
 
SwissProt: Q9JHX4 Rat
 
Unigene: 599762 Human
 
Unigene: 655983 Human
 
Unigene: 336851 Mouse
 
 
 
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
半胱氨酸蛋白酶-8，在凋亡执行阶段起中心作用。
产品图片
Sample:
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 43 kD
Sample:
Stomach(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Urinary bladder(Mouse) Lysate at 40 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 55'53D
Sample:
293T-UV(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41’43’55 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.