货号：BW001-04
 
氨基酸序列：MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
 
共171个氨基酸残基，其中N末端的31个氨基酸残基（下划线）为His-tag及酶切位点。其余部分与小鼠全长脂联素的108-248位的氨基酸序列完全相同。
 
规格：0.1mg (BCA法测定)
来源： 大肠杆菌(E. coli)
 
产品形态：冻干粉（无菌过滤，0.5mg/ml脂联素，PBS缓冲液）
 
溶解：加0.2ml去离子水，室温下彻底溶解，最终浓度为0.5mg/ml。
 
保存条件： -20℃保存。建议溶解后分装保存，避免反复冻融。溶解后可以暂时保存在4℃至两周。
 
应用： ELISA、Western blotting、细胞培养、动物研究。
 
有效期：见包装。
 
质控检测：BCA-测定蛋白浓度
SDS PAGE-测定蛋白纯度
 
注意：本产品仅用于科研。

Mouse Adiponectin Globular, Recombinant Protein

AA sequence: MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
Total 171AA. N-terminal underlined amino acids are His-tag and the protease cleavage site (31AA). The AA sequence is homologous to the 108-248 amino acid sequence of the mouse full length adiponectin.
Size: 0.1 mg (determined by BCA method)
Source: E. coli
Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer
Reconstitution: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Applications: ELISA, Western blotting, cell culture and/or animal studies
Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
Quality Control Test: BCA - to determine content of the protein
SDS PAGE - to determine purity of the protein
Note: For research use only

Introduction:
Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (LMW, also called trimer) forms. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.

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• Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002; 277:19521-19529.
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