BamHI
产品名称： BamHI
英文名称： BamHI
产品编号： HZ00132
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
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- 邮箱 : www.shzbio.net
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BamHI
产品描述
使用说明
1。高浓度may result in星的酶活性。2。do not use for long BSA的培养。3。to obtain 100% activity，BSA should be added to the 1X和杆端反应concentration of 100μg／ml。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。
网站g↑gatcc recognition
带源大肠杆菌的应变，解淀粉芽孢杆菌检测基因BamHI from the h
我们assayedλDNA
优化10毫米的Tris HCl缓冲溶液（pH 7.6毫米（°C）；10 50毫米氯化钠；氯化镁；1毫米DTT。+ BSA。
37°C温度的优化
10毫米的存储条件的Tris-HCl（pH 7.6）0.1毫米，50毫米氯化钠；EDTA；100μg／ml）；1毫米DTT；50%的甘油。
ligations 50倍后的酶overdigestion with more than 90% of the DNA碎片重新ligated and can be。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of 16 hours for 40 UA听说是八周的酶在37°C。
甲基化或甲基化效应（not by坝ggatcc gmatc）。
热灭活的灭活酶是由孵化在150度为20分钟。
兼容的末端bcli bsp143i mboi bgl II，，，，psui。
使用说明
1.High enzyme concentration may result in star activity. 2.Do not use BSA for long incubation. 3.To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | G↑GATCC |
| Source | E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H |
| Assayed on | Lambda DNA |
| Optimal buffer | 10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT. +BSA. |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. |
| Ligations | After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Not blocked by Dam methylation (GmATC) GGATCC. |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| 兼容的末端 | BclI, BglII, Bsp143I, MboI, PsuI. |