25-羟基-维生素D2/D3诊断试剂盒（串联质谱法LC-MS/MS）-临床检测试剂-试剂-生物在线
北京祥生兴业科技有限公司
25-羟基-维生素D2/D3诊断试剂盒（串联质谱法LC-MS/MS）

25-羟基-维生素D2/D3诊断试剂盒（串联质谱法LC-MS/MS）

商家询价

产品名称： 25-羟基-维生素D2/D3诊断试剂盒（串联质谱法LC-MS/MS）

英文名称： MassChrom® Reagent kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma

产品编号： 62000

产品价格： 0

产品产地： 德国

品牌商标： MassChrom®

更新时间： null

使用范围： null

北京祥生兴业科技有限公司
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进入展台

  • Isotopically labelled internal standard

  • Multilevel calibrators

  • Online sample preparation

  • Upgrade available for the dermination of 3-epi-25-OH-Vitamin D3/D2

Vitamin D has been widely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects.

25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-Vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation. Vitamin D2 is easier to produce synthetically and, where prescribed, its monitoring is also necessary.

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. The 3PLUS1® multilevel calibrators offer additional precision.

The upgrade to MassChrom® 25-OH-vitamin D3/D2 analysis allows for the separation of the main metabolites of vitamins D3 and D2, 25-Hydroxycholecalciferol and 25-Hydroxyergocalciferol, from their epimeric forms 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2, hence allowing for separate quantification by LC MS/MS.

Standard method

Upgrade for determination of
diastereomeric forms

Specifications:

Linearity: up to 250 µg/l
Limit of quantification: 25-OH-Vitamin D3: 2.0-3.0 µg/l 3-epi-25-OH-Vitamin D3: 2.0 µg/l
25-OH-Vitamin D2: 1.0-2.2 µg/l 3-epi-25-OH-Vitamin D2: 1.0 µg/l
Intra-assay: CV < 5 %
Inter-assay: CV < 7 %
Recovery: 25-OH-Vitamin D3: 93-104 % 3-epi-25-OH-Vitamin D3: 102-106 %
25-OH-Vitamin D2: 91-101 % 3-epi-25-OH-Vitamin D2: 104-105 %
Run time: 25-OH-Vitamin D3/D2: 5 min 3-epi-25-OH-Vitamin D3/D2: 8.5-10 min


HPLC parameters

Injection volume: 10 to 50 µl

Pump A delivers 100 % Mobile Phase A for loading the trap column.

Flow: 1.00 ml/min

Pump B delivers 100 % Mobile Phase B for separating analytes on the analytical column.

Flow: 0.60 ml/min

The trap column and the analytical column are linked to the mass spectrometer through a 6-port valve.


LC-MS/MS parameters

Ionisation: APCI positive
MS/MS-mode:

 

  • Place 100 µl sample into a reaction vial.
  • Add 25 µl Precipitation Reagent.
  • Add 200 µl Internal Standard and vortex 20 s.
  • Incubate 10 min at +4 °C.
  • Centrifuge 5 min at 15000 g.
  • Transfer 200 µl supernatant into an autosampler vial.

    Order no. Product
    62000 MassChrom® Reagent kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma
    For 200 analyses
    62000/1000 MassChrom® Integrated kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma,
    For 1000 analyses, complete with:
    Analytical column
    Trap-column
    3PLUS1® Calibrators
    MassCheck® Controls
    62000/1000/F MassChrom® Integrated kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma,
    For the automated sample preparation with 96 well filter plates.
    For 1000 analyses, complete with:
    Analytical column
    Trap-column
    3PLUS1® Calibrators
    MassCheck® Controls
    Components available separately:
    62001 Mobile Phase A, 1000 ml
    62002 Mobile Phase B, 1000 ml
    62009 Rinsing Solution, 1000 ml
    62003 Precipitation Reagent, 5 ml
    62004 Internal Standard, 40 ml
    3006 Reaction vials, 100 pcs./td>
    Multilevel Calibrators and Startup Accessories:
    62028 3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 4 x 1 ml
    62039 6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 7 x 1 ml
    62110 Trap column, 1 pc.
    62100 Analytical column, 1 pc.
    62015 Tuning Mix 25-OH-Vitamin D3/D2, 2 ml
    15010 PEEK prefilter housing, 1 pc.
    15011 PEEK-encased Prefilter, 2 µm, 5 pcs.
    Chromsystems MassCheck® Controls (lyoph.):
    0221 MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Bi-Level (I+II), 2 x 5 x 1 ml
    0222 MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level I, 5 x 1 ml
    0223 MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level II, 5 x 1 ml
    0256 MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level III, 5 x 1 ml
    Accessories for sample preparation with 96 well filter plates:
    62057 96 Well filter plates, 3 pcs.
    62058 Collection plates, 3 pcs.
    62059 Pierceable adhesive seals, 3 pcs.
    Upgrade accessories for the analysis of diastereomeric forms of 25-OH-Vitamin D3/D2:
    62044 Internal Standard Mix (for 200 analyses), 40 ml
    62029 3PLUS1® Multilevel Serum Calibrator Set 3-epi-25-OH-D3/D2 and 25-OH-D3/D2 (lyoph.), 4 x 1 ml
    0310 MassCheck® 3-epi-25-OH-D3/D2 and 25-OH- D3/D2 Serum Control, Bi-Level (I + II) (lyoph.), 2 x 5 x 1 ml
    0311 MassCheck® 3-epi-25-OH-D3/D2 and 25-OH- D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml
    0312 MassCheck® 3-epi-25-OH-D3/D2 and 25-OH-D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml
    62120 Analytical column for determination of epimers (equilibrated with test chromatogram), 1 pc.
    62110/Epi Trap column for determination of epimers (equilibrated with test chromatogram), 1 pc.
    62016 Tuning Mix, contains 25-OH-Vitamin D3/D2 and ISTD 1, 1 ml