XmaI内切酶
中文名称：XmaI内切酶
英文名称：XmaI
产品规格：100U
储存条件：冷冻（-20℃）
技术规格
| Unit definition |
One unit of the enzyme is the amount required to hydrolyze 1μgof Adenovirus -2 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites |
C↑CCGGG |
| 来源 |
E.coli strain,that carries the cloned gene XmaI from Xanthomonas malvacearum |
| Assayed on |
Adenovirus -2 DNA |
| Optimal buffer |
Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature |
37℃ |
| Storage conditions |
20 mM Tris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations |
After 3-fold overdigestion with enzyme 95%of the DNA fragments can be ligated.Of these 90%can be recut. |
| Non-specific hydrolisis |
No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 3 u.a.of enzyme for 16hoursat 37℃ |
| Methlation effect |
Methylation sensitivity not tested |
| Thermal inactivation |
enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends |
Cfr9I,PspAI,SmaI^,TspMI,XmaCI |
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
