内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体
产品名称： 内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体
英文名称： Visfatin
产品编号： 0272
产品价格： null
产品产地： 上海
品牌商标： 雅吉
更新时间： null
使用范围： WB IHC-P IHC-F IF
上海雅吉生物科技有限公司
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| 中文名称 | 内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体 |
| 别 名 | pre-B-cell colony-enhancing factor 1; PBEF1 protein; AI480535; 1110035O14Rik; AI314458; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Visfatin; NAMPT_HUMAN. |
| 研究领域 | 心血管 神经生物学 糖尿病 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, Horse, Monkey, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PBEF:301-400/491 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]. Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. Similarity: Belongs to the NAPRTase family. SWISS: P43490 Gene ID: 10135 Database links: Entrez Gene: 10135 Human Entrez Gene: 59027 Mouse Entrez Gene: 297508 Rat Omim: 608764 Human SwissProt: P43490 Human SwissProt: Q99KQ4 Mouse SwissProt: Q80Z29 Rat Unigene: 489615 Human Unigene: 202727 Mouse Unigene: 203508 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 一种新的脂肪因子-visfatin是新近发现的主要由人和小鼠内脏脂肪组织分泌的一种脂肪细胞因子,其结构与pre-B细胞集落增强因子相似.它能够发挥类似胰岛素的作用,与Ⅱ型糖尿病相关联,降低血糖,促进糖摄取,可结合并活化胰岛素受体,激活胰岛素信号通路.内脏脂肪因子-Visfatin与肥胖密切相关并能够促进脂肪细胞的分化,还能促进血管平滑肌细胞成熟. Visfatin的表达受炎症反应因子和多种激素的调节.PBEF-1可能是联系机体糖脂代谢的重要分子,它的发现可为揭示糖尿病与肥胖的发生发展机制提供新的研究思路,为代谢综合征的治疗提供新方案. |
| 产品图片 |  Sample:Heart (Mouse) Lysate at 40 ug Primary: Anti-Visfatin (bs-0272R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Lane1:Brain(Rat) Lysate at 30 ug Lane2:Liver (Rat) Lysate at 30 ug Primary: Anti-VISFATIN-1 (bs-0272R) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 55kD Observed band size : 54kD  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(bs-0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(bs-0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |