PalAI

附带条件

酶的一个单位是在37℃的1小时内，在50μL的总反应体积中水解1μgλDNA所需的量。

识别位点GG-CGCGCC

源假单胞菌产碱杆菌BS17

λDNA检测

最优缓冲区Y？（33毫米醋酸三乙酯（pH为7.9，在25°C）；10毫米醋酸镁；66毫米醋酸钾；1毫米DTT）。

最佳温度37°C

贮藏条件为10 mM TrI HCl（pH 7.5）；100 mM氯化钾；0.1 mM EDTA；7 mM 2-巯基乙醇；200μg/ml牛血清白蛋白；50%甘油；在-20℃储存。

2倍以上消化超过90%的DN**段的结扎可以结扎和重新缝合。

在37℃下，用1μgλDNA和2μA酶孵育16小时后，检测到非特异性Hyrasists无非特异性的活性。

CG甲基化阻断甲基化效应。

在65℃下孵育20分钟，使失活酶失活。

Campatible Ends AscI，SgsI

技术规格

 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Recognition sites	GG↑CGCGCC
Source	Pseudomanas alcaligenes BS17
Assayed on	Lambda DNA
Optimal buffer	Y?(33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature	37°C
Storage conditions	10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations	After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methlation effect	Blocked by CG methylation.
Thermal inactivation	enzyme is inactivated by incubation at 65°C for 20 mins.
Campatible Ends	AscI, SgsI