抗体来源 Rabbit 

克隆类型 Polyclonal 

交叉反应 Human, Rat,  (predicted: Mouse, ) 

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 19/30/37kDa 

细胞定位 细胞浆  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

 

Function:

Important for the overall degradation of proteins in lysosomes.

 

Subunit:

Dimer of a heavy and a light chain linked by disulfide bonds.

 

Subcellular Location:

Lysosome.

 

Similarity:

Belongs to the peptidase C1 family.

 

SWISS:

P06797

 

Gene ID:

1514

 

Database links:

Entrez Gene: 1514 HumanEntrez Gene: 13039 MouseEntrez Gene: 25697 RatOmim: 116880 HumanSwissProt: P07711 HumanSwissProt: P06797 MouseSwissProt: P07154 RatUnigene: 726015 HumanUnigene: 930 MouseUnigene: 1294 Rat

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

 

产品图片

Sample: A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40 kD Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (bs-1508R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kD Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(bs-1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(bs-1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining