NI SEPHAROSE 6FF-色谱介质-耗材-生物在线
上海基星生物科技有限公司
NI SEPHAROSE 6FF

NI SEPHAROSE 6FF

商家询价

产品名称： NI SEPHAROSE 6FF

英文名称： NI SEPHAROSE 6FF

产品编号： 17-5318-01

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
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17-5318-01 NI SEPHAROSE 6FF, 25 ML
17-5318-02 NI SEPHAROSE 6FF, 100 ML
17-5318-03 NI SEPHAROSE 6FF, 500 ML
17-5318-06 NI SEPHAROSE 6 FF 5 ML
17-5438-01 MABSELECT SURE 25 ML
17-5438-02 MABSELECT SURE 200 ML
17-5448-01 Capto Blue 25 ml
17-5474-01 MabSelect SuRe LX 25 ML
17-5474-02 MabSelect SuRe LX 200 ML
17-6002-35 IMMUNOPRECIP STARTER PACK
18-1022-29 HB AFFINITY CHROMATOGRAPHY
18-1037-46 ANTIBODY PURIFICATION HB
28-9355-97 Dextrin Sepharose High Perform
28-9355-98 Dextrin Sepharose High Perform
28-9356-00 StrepTactin Sepharose High Per
28-9805-82 MembraneProteinPurificationKit
17-5468-15 HiScreen Capto SP ImpRes
17-5470-15 HiScreen Capto Q ImpRes
17-5474-15 HiScreen MabSelect SuRe LX

Ni Sepharose™ 6 Fast Flow - Scale-up Purification

  • Designed for scaling up purification of histidine-tagged* proteins.
  • Suitable for gravity-flow purification and multiwell plate screening.
  • High protein binding capacity.
  • Negligible leakage of Ni2+ ions.
  • Compatible with a wide range of reducing agents, detergents, and other additives

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Ni Sepharose™ 6 Fast Flow consists of 90-μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged protein. The ligand density of Ni Sepharose 6 Fast Flow ensures high binding capacity while leakage of Ni2+ ions is negligible, which ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications. The high flow rate property of the Sepharose 6 Fast Flow matrix makes it ideal for scaling up purposes.

 

Ni Sepharose 6 Fast Flow is designed for scaling up purification of histidine-tagged proteins, gravity-flow purification, and multiwell plate screening.

 


 

Ni Sepharose™ 6 Fast Flow - Scale-up Purification

Technical Information


For more information on these products and the complete range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.

 

Ni Sepharose 6 Fast Flow is supplied preswollen in 20% ethanol. The medium is also available prepacked in 1-ml and 5-ml HisTrap FF and 20-ml HisPrep FF 16/10 columns, see HisPrep™ FF 16/10 Column - Easy Scale-up Purification, 1-ml and 5-ml HisTrap FF crude columns, see HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates, His MultiTrap FF 96-well plates, see His MultiTrap 96-Well Filter Plates - High-throughput Screening, and in His GraviTrap columns, see His GraviTrap/His GraviTrap Kit - Gravity-flow Purification.

 

IMAC Sepharose 6 Fast Flow, see IMAC Sepharose 6 Fast Flow Media/HiTrap IMAC FF Columns/HiPrep IMAC FF 16/10 Column/HiScreen IMAC FF Column, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

 

* For licensing information, see Legal Info.

 

TECHNICAL SPECIFICATIONS
Matrix  Highly crossed-linked 6% agarose, precharged with Ni2+ 
Average particle size  90 μm 
Dynamic binding capacity*  Approx. 40 mg histidine-tagged protein/ml medium 
Metal ion capacity  Approx. 15 μmol Ni2+/ml medium 
Compatibility  during use  Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information. 
Chemical stability†  For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1 M NaOH, 70% acetic acid
30 min tested: 30% 2-propanol
1 h tested: 2% SDS 
pH stability†  2-14 (short term), 3-12 (long term) 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* Protein binding capacity is protein-to protein dependent
† Ni2+-stripped medium

 

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