详细配制内容及参数资料

1.1 特点

l可以做更准确的药代动力学和毒物学的研究

l更清楚准确的反应了体内血脑屏障的特性

l模拟重要的内皮细胞与星形胶质细胞之间的作用

l用电力方法检测了血脑屏障的完整性

l可以用真实的细胞做实验

l比现存的静态模型更能形成紧密的连接

l降低药物研发的成本

1.2 配置及参数资料

TEER检测系统CARTRIDGE底板

4个DIV-BBB盒子检测软件电缆线

DIV-BBB?管腔

经毛细管孔径：0.2um

中空纤维数量：19

腔内总体积：0.0123in³= 0.202cm³

腔内表面积：2.09 in³= 13.5cm²

腔外体积：0.070 in³= 1.15cm³

腔外中空纤维总面积：3.50 in³= 22.6cm²

TEER检测系统

TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法，此外，电阻抗值（＞1000 Ω-cm²）也非常接近体内数值。TEER检测系统同时配带了所需检测软件、电线和衬板，可供4个DIV-BBB管腔同时使用。其中检测软件可以自定义设置泵的流速、频率、波形等参数。其它特性及优点见下表：

脉动泵

脉搏率和作用周期完全模拟体内血液动力学设计，贴近体内环境，试验准确，脉搏率可设置在30-120/分范围内。其特性和优点见下：

电脑配置最低要求

?IBM兼容电脑，400MHz或者更快

?Window 2000或者更高系统

?128MB存储器（推荐256MB）

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About Flocel

Every year millions of dollars are spent by pharmaceutical companies to develop and test drugs using in vitro models of the Blood-Brain Barrier (BBB). Many of these cases end up not working in vivo.CleveMedhas licensed technology fromThe Cleveland Clinic Foundation and formed Flocel Inc, to manufacture a practical in vitro model that more accurately reflects the BBB characteristics. Flocel's dynamic in vitro blood-brain barrier (DIV-BBB) technology helps researchers in the United States and Europe conduct in vitro studies.

Flocel also offers atesting service for BBB permeability compounds. To learn more about our testing services, clickhere.

Introduction to the Blood-Brain Barrier

TThe blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. The BBB is formed by brain capillaries that look like simple tubes of endothelial cells. Experimental and clinical evidence suggests that the BBB maintains the chemical environment for neuronal function and protects the brain from harmful substances. The blood-brain barrier is therefore crucial for complex issues such as drug delivery, pathogenesis of chronic neurological diseases, and issues related to bio-defense. We have developed a practical in vitro model that accurately reflects BBB characteristics.

Benefits of Flocel’s in vitro BBB Model

In contrast to other available models of the blood-brain barrier, Flocel's Dynamic In vitro Blood-Brain Barrier (DIV-BBB) respects the anatomical aspects of the in situ endothelial cell-astrocyte interactions and replicates the physiological levels of shear stress to which in situ endothelial cells are exposed. The DIV-BBB allows formation of physiological transendothelial resistance, and formation of gap junctions that can be easily visualized at the electron microscope or determined experimentally with the use of tracers.

Mission

Flocel Inc. is a biotechnology company dedicated to innovation towards helping the research community better conduct and advance in vitro studies.

In vitro BBB Technology

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