Overview

Product name
Superoxide Dismutase Activity Colorimetric Assay Kit


Product overview
The sensitive Superoxide Dismutase (SOD) Activity Assay Kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. The lower limit for this kit is 0.1 U/ml of SOD activity.


Notes
Sample preparation.



1. Blood samples: 



Collect blood using citrate or EDTA. Centrifuge at 1,000 x g for 10 min at 4°C. Transfer the plasma layer to a new tube without disturbing the puffy layer and store at -80°C until ready for analysis. Remove the puffy layer from the red cell pellet. Resuspend the erythrocytes in 5x volume of ice cold distilled water and centrifuge at 10,000 x g to pellet the erythrocyte membranes. Store the supernatant at -80°C until ready for analysis. Plasma can be diluted approx. 3-10x and the red cell lysate diluted approx. 100x prior to SOD assay.



2. Tissue and cell samples: 



Tissue should be perfused with PBS or 150mM KCl to remove any red blood cells. Homogenize tissue or lyse cells in ice cold 0.1M Tris/HCl, pH 7.4 containing 0.5 % Triton X-100, 5mM ß-ME, 0.1mg/ml PMSF. Centrifuge the crude tissue homogenate/cell lysate at 14000 x g for 5 minutes at 4°C and discard the cell debris. The supernatant contains total SOD activity from cytosolic and mitochondria.

 


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at +4°C.



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
SOD Assay Buffer
            
WM
            
1 x 20ml
        
        

            
SOD Dilution Buffer
            
NM
            
1 x 10ml
        
        

            
SOD Enzyme Solution
            
Green
            
1 x 20µl
        
        

            
WST Solution
            
Red
            
1 x 1ml
        
    


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