Product Description
Poly-L-Lysine (PLL), a synthetic compound, is a highly positively charged amino acid chain that enhances cell adhesion by altering surface charges on the culture substrate [1]. It is commonly used as a coating agent to promote cell adhesion in culture. In addition to promoting cell adhesion, PLL surface treatments improve the survival of many primary cells in culture and support neurite outgrowth. This solution is provided as stock solution and contains polymers in the 70,000 - 150,000 kDa range.
Concentration
10 mg/ml, sterile-filtered.
Storage Conditions
Product is stable for at least 6 months from the date of receipt when stored at 2 - 8° C. Keep sterile.
Applications
Substrate for cell culture adhesion. Optimal conditions for attachment must be determined for each cell line and application. Recommended concentration for normal human cell attachment is 2 μg/cm2.

Specifications
1. Functional Assay: Tested for ability to promote attachment of normal human cells.
2. Sterility Testing: No bacterial or fungal growth detected after incubation at 37° C for 14 days following USP XXIV Chapter 71 sterility testing.
3. No mycoplasma contamination detected by PCR.
4. Endotoxin concentration ≤ 20 EU/ml by LAL assay.