HITRAP RPROTEIN A FF-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
HITRAP RPROTEIN A FF

HITRAP RPROTEIN A FF

商家询价

产品名称： HITRAP RPROTEIN A FF

英文名称： HITRAP RPROTEIN A FF

产品编号： 17-5079-01

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
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17-5079-01 HITRAP RPROTEIN A FF, 5X1ML
17-5079-02 HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01 HITRAP RPROTEIN A FF, 1X5ML
17-5080-02 HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01 HITRAP IGM PURIFICATION 5X1ML
17-5111-01 HITRAP IGY PURIFICATION 1X5 ML
17-5112-01 HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01 GSTRAP FF, 5X1ML
17-5130-02 GSTRAP FF, 2X1ML
17-5130-05 GSTRAP FF, 100 X 1 ML
17-5131-01 GSTRAP FF, 1X5ML
17-5131-02 GSTRAP FF 5 X 5 ML
17-5131-05 GSTRAP FF, 100 X 5 ML
17-5143-01 HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02 HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01 HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01 HITRAP DEAE FF 5 X 5 ML
17-5155-01 HITRAP CM FF, 5 X 5 ML
17-5156-01 HITRAP Q FF, 5 X 5 ML
17-5157-01 HITRAP SP FF, 5 X 5 ML
17-5158-01 HITRAP Q XL, 5 X 1 ML
17-5159-01 HITRAP Q XL, 5 X 5 ML
17-5160-01 HITRAP SP XL, 5 X 1 ML
17-5161-01 HITRAP SP XL, 5 X 5 ML
17-5162-01 HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01 HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01 HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01 HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01 HITRAP PHENYL HP, 5 X 5 ML
17-5196-01 HITRAP OCTYL FF, 5 X 5 ML
17-5197-01 HITRAP BUTYL FF, 5 X 5 ML
17-5247-01 HISTRAP HP 5 X 1 ML
17-5247-05 HISTRAP HP 100 X 1 ML
17-5248-01 HISTRAP HP 1 X 5 ML
17-5248-02 HISTRAP HP 5 X 5 ML
17-5248-05 HISTRAP HP 100 X 5 ML
17-5255-01 HISTRAP FF 5 X 5 ML
17-5255-02 HISTRAP FF 100 X 5 ML
17-5281-01 GSTRAP HP 5 X 1 ML
17-5281-05 GSTRAP HP 100 X 1 ML
17-5282-01 GSTRAP HP 1 X 5 ML
17-5282-02 GSTRAP HP 5 X 5 ML
17-5282-05 GSTRAP HP, 100 X 5 ML
17-5286-01 HISTRAP FF CRUDE 5X5 ML
17-5286-02 HISTRAP FF CRUDE 100X5 ML
17-5319-01 HISTRAP FF 5 X 1 ML
17-5319-02 HISTRAP FF 100 X 1 ML
17-5441-22 HITRAP CAPTO S, 5X1 ML
17-5441-23 HITRAP CAPTO S, 5X5 ML
17-5468-51 HiTrap Capto SP ImpRes 5x1mL
17-5468-55 HiTrap Capto SP ImpRes 5x5mL
17-5470-51 HiTrap Capto Q ImpRes 5x1mL
17-5470-55 HiTrap Capto Q ImpRes 5x5mL
17-6002-33 HITRAP IEX SELECTION KIT
18-1142-75 REC PROTEIN PURIF HANDBOOK
28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77 HISTRAP FF CRUDE KIT
28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML

HiTrap™ rProtein A FF Columns

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources.
  • Recombinant Protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification

HiTrap™ rProtein A FF Columns

Technical Information


The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

 

HiTrap™ rProtein A 1 ml and 5 ml columns are packed with 1 ml and 5 ml of rProtein A Sepharose™ Fast Flow, respectively.

 

TECHNICAL SPECIFICATIONS
Column dimensions (i.d.x.h)  0.7 x 2.5 cm (1 ml) 
  1.6 x 2.5 cm (5 ml) 
Ligand  Recombinant protein A (E. coli) 
Ligand coupling method  Epoxy activation, thioether coupling 
Ligand density  » 6 mg rProtein A/ml medium 
Matrix  Highly cross-linked agarose, 4% 
Average particle size  90 μm 
Binding capacity  » 50 mg human IgG/ml medium 
Dynamic binding capacities †  23 mg mouse monoclonal IgG2a per ml gel 
  12 mg mouse monoclonal IgG1 per ml gel 
  11 mg monoclonal humanised IgG4 per ml gel 
Maximum back pressure  3 bar, 0.3 MPa, 44 psi 
Maximum flow rate ‡  4 ml/min (624 cm/h) for 1 ml column 
  20 ml/min (600 cm/h) for 5 ml column 
Recommended working flow rate  1 ml/min (156 cm/h) for 1 ml column 
  5 ml/min (150 cm/h) for 5 ml column 
Chemical stability  All commonly used buffers 
pH stability  3-10 (working), 2*-11 (short term) 
Recommended flow rate  1 ml/ min (1-ml column)
5 ml/min (5-ml column) 
Maximum flow rate  4 ml/min (1-ml column)
20 ml/min (5-ml column) 
Temperature stability   
Regular use  +4-40°C 
Storage  +4-8°C 
Storage buffer  20% ethanol 
Storage  20% ethanol 
Storage temperature  4°C to 8°C 
* pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.

 
† Capacity of HiTrap™ rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

 
‡ Room temperature, aqueous buffers

 
Column: HiTrap™ rProtein A 1 ml. Flow rate: 1 ml/min.

 
Sample: cell culture supernatants.

 

 

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SDS-PAGE on PhastSystem™ using PhastGel™ Gradient 10-15, silver stained

 

Lane 1:   Low Molecular Weight Calibration Kit (LMW)
Lane 2:   Starting material, diluted 10×
Lane 3:   Flowthrough pool
Lane 4:   Eluted IgG2b pool

 

Purification of mouse IgG2b from ascites on HiTrap™ rProtein A FF using a syringe.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=12887