β-淀粉酶检测试剂盒适用于麦芽粉中β-淀粉酶的特异性测定和分析。

Colourimetric method for the determination of β-Amylase in 

cereal grains, malt, food, beverages and fermentation products

Principle:

                          (β-amylase)

(1) G3-β-PNP + H2O → G2 + G-β-PNP

                     (β-glucosidase)

(2) G-β-PNP + H2O → D-glucose + PNP

                 (alkaline solution)

(3) p-Nitrophenol → phenolate ion (yellow colour)

Note: PNP = 4-nitrophenol

Kit size:                            100 / 200 assays

Method:                            Spectrophotometric at 400 nm

Reaction time:                  ~ 10 min

Detection limit:                 0.05 U/mL of sample solution

Application examples: 

Cereal flours, malts and other materials

Method recognition:     

Modification of RACI (Standard Method)

 Advantages
Very cost effective 
All reagents stable for > 2 years as supplied 
Only enzymatic kit available 
Very specific 
Simple format 
Rapid reaction 
Mega-Calc software tool is available from our website for hassle-free raw data processing 
Standard included