GSTPrep FF 16/10
产品名称: GSTPrep FF 16/10
英文名称: GSTPrep FF 16/10
产品编号: 28-9365-50
产品价格: 0
产品产地: 美国
品牌商标: GE
更新时间: null
使用范围: null
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28-9365-50 | GSTPrep FF 16/10 | 1 ×20 ml |
GSTPrep™ FF 16/10 Column - Easy Scale-up Purification
Technical Information
GSTPrep™ FF 16/10 for fast and easy scale-up purification of GST-tagged recombinant proteins.
GSTPrep™ FF 16/10 are 20-ml HiPrep™ columns prepacked with Glutathione Sepharose™ 4 Fast Flow for scale-up purification of recombinant GST-tagged proteins, other glutathione S-transferases, and glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow in combination with HiPrep™ column hardware provides excellent flow properties.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165074
TECHNICAL SPECIFICATIONS | |
Medium | Glutathione Sepharose™ 4 Fast Flow |
Column volume | 20 ml |
Column dimensions | 1.6 × 10 cm |
Recommended flow rate* | 1-10 ml/min (30-300 cm/h) |
Max. flow rate* | 10 ml/min (300 cm/h) |
Max. pressure over the packed bed during operation* | 1.5 bar (0.15 MPa, 22 psi) |
Column hardware pressure limit | 5 bar (0.5 MPa, 73 psi) |
Storage | 20% ethanol |
Storage temperature | 4°C to 30°C |
* H2O at room temperature. Note: Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider, especially during sample loading and elution. |