Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody-抗体-抗体-生物在线
上海雅吉生物科技有限公司
Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody

Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody

商家询价

产品名称: Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody

英文名称: Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody

产品编号: K5743

产品价格: null

产品产地: 上海

品牌商标: 雅吉生物

更新时间: null

使用范围: WB, IF, ICC

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来源用途交叉反应性理论分子量实际分子量
RabbitWB, IFH350kDa350kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WBIPIFIHCICCFCChIPChIP-seq
1:500-1:2000-1:50-1:200-----

抗体详细信息如下::

About this Antibody
NamePhospho-ATM (Ser1981) Rabbit Polyclonal Antibody
CategoryRabbit Polyclonal Antibody (pAb); Primary antibody
IsotypeIgG
Purification methodAffinity purification
Positive samples293T
Cellular locationCytoplasmic vesicle, Nucleus
Customer validation-
About the Immunogen
ImmunogenA synthetic phosphorylated peptide around S1981 of human ATM (NP_000042.3).
SequenceEGSQS
Gene ID472
Swiss ProtQ13315
SynonymsATM; AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1; serine-protein kinase ATM
CategoryRegulation of Apoptosis; G1/S Checkpoint; G2M/DNA Damage Checkpoint; Mitochondrial Control of Apoptosis
BackgroundAtaxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.

包装清单:

产品编号产品名称包装
AF5743Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody50μl
AZ050Western一抗稀释液50ml
说明书1份

保存条件:
Phospho-ATM (Ser1981) Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

代表性图片:

Fig. 1. Western blot analysis of extracts of 293T cells, using Phospho-ATM-S1981 antibody at 1:2000 dilution. 293T cells were treated by UV for 15-30 minutes. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.Fig. 2. Immunofluorescence analysis of MCF-7 cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.
Fig. 3. Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.Fig. 4. Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.