STEMvision™ is an instrument designed specifically for imaging and counting colonies in the hematopoietic colony-forming unit (CFU) assay using MethoCult™ media and meniscus-free SmartDish™ cultureware. STEMvision™ replaces the need to manually count colonies using a microscope.

Three new STEMvision™ Analysis Packages have been designed for counting hematopoietic colonies produced by erythroid, myeloid and multi-potential progenitor cells (CFU-E, BFU-E, CFU-G/M/GM and CFU-GEMM) in 14-day CFU assays of cord blood, bone marrow and mobilized peripheral blood cells cultured in MethoCult™ Optimum. A fourth Analysis Package is also available for counting the total number of colonies (without classification of CFU subtypes) in 7-day CFU assays of cord blood cells cultured in MethoCult™ Express.

Users simply load a SmartDish™ into the STEMvision™ instrument, acquire an image of the entire culture dish and sophisticated analysis software is then used to automatically identify and classify the hematopoietic colonies. The results can be reviewed visually and exported to databases for reporting and archiving purposes.

Images of CFU assay cultures are acquired in approximately 30 seconds. Image analysis and colony counting are performed in approximately 3 minutes. Alternatively, multiple SmartDish™ plates can be imaged sequentially, and batch analysis performed at a later time (e.g. overnight).

Several purchase and leasing options are available. Please contact us for further information.

Application: Colony assays & quantification
Product Type: Specialized cell culture media
Recommended for: Automated and standardized counting of CFU assays of human hematopoietic cells.
Components: • STEMvision™ Instrument (dimensions: 478 mm W x 335 mm D x 347 mm H)
• Computer and monitor
• One- or two-year warranty
• Installation and training
Accessory Products: • STEMvision™ Human Cord Blood 7-Day Analysis Package(Catalog #22001)
• STEMvision™ Human Cord Blood 14-Day Analysis Package (Catalog #22002)
• STEMvision™ Human Bone Marrow 14-Day Analysis Package (Catalog #22003)
• STEMvision™ Human Mobilized Peripheral Blood 14-Day Analysis Package (Catalog #22004)
• MethoCult™ Express (Catalog #04437/04447)
• MethoCult™ H4034 Optimum (Catalog #04034/04044)
• SmartDish™ (Catalog #27301)
• One-Year Warranty for STEMvision™ Instrument (Catalog #22201)
• Preventative Maintenance (No Warranty) for STEMvision™ Instrument (Catalog #22202)
• One-Year Warranty with Preventative Maintenance for STEMvision™ Instrument (Catalog #22203)
• Preventative Maintenance (Active Warratny) for STEMvision™ Instrument (Catalog #22209)
Area of Interest: Cord blood banking, Hematopoietic stem cell research, Transplantation
Cell Type: Hematopoietic stem & progenitor cells
Medium Type: Methylcellulose-based
Popular Product Line: MethoCult
Intended Use Statement: For Research Use Only. Not Intended for Human or Animal Diagnostic or Therapeutic Uses.