pET-11d(+)图谱序列抗性标签酶切位点
产品名称: pET-11d(+)图谱序列抗性标签酶切位点
英文名称: pET-11d(+)图谱序列抗性价格Biovector质粒库
产品编号: 39597928973
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector中国质粒载体菌株细胞
更新时间: null
使用范围: null
pet11d载体基本信息
出品公司: EMD Biosciences(Novagen) 别名: pET11d, pet 11d 质粒类型: 大肠杆菌蛋白表达 表达水平: 高 克隆方法: 多克隆位点,限制性内切酶 载体大小: 5674bp 5' 测序引物: T7 5' 测序引物序列: 5'-TAATACGACTCACTATAGGG-3' 载体标签: N-T7 载体抗性: Ampicillin 备注: Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.产品目录号: 69439-3 稳定性: 瞬时表达 Transient 组成型: 组成型 Constitutive 病毒/非病毒: 非病毒 pet11d载体质粒图谱和多克隆位点信息
Feature Name
Start
End
T7_terminator 316 188 T7_Terminal_primer 256 274 T7_leader 358 326 T7_transl_en_RBS 382 366 lacO 427 400 T7_promoter 445 427 pBRrevBam_primer 548 529 lacI 823 1914 tet (576 - 1190) 1973 2587 ROP 3226 3417 pGEX_3_primer 3433 3411 Ampicillin 5466 4606 AmpR_promoter 5536 5508
ORF
Start
End
ORF frame 3 582 1082 ORF frame 1 955 1914 ORF frame 1 2392 1697 ORF frame 2 1970 2587 ORF frame 2 5466 4606
Enzyme Name
CutClaI 24 HindIII 29 BamHI 319 NheI 350 NcoI 355 XbaI 394 BglII 460 BclI 1196 ApaI 1393 HpaI 1688 EagI 2250 NruI 2285 MscI 2757 PstI 4924 AatII 5601 EcoRI 5672 pet11d载体简介
The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.
pet11d载体序列
LOCUS pET11d 5674 bp DNA circular SYN
DEFINITION pET11d
ACCESSION
KEYWORDS
SOURCE