Description
VELOCITY is a fast, proofreading DNA polymerase from archaeal origin which generates blunt-ended amplicons. Its high thermostability
combined with its 5’-3’ DNA polymerase and 3’-5’ proofreading exonuclease activities makes VELOCITY an ideal enzyme for all PCR
applications. Indeed, VELOCITY possesses an error-rate of 4.4 x 10-7, providing a 50-fold higher fidelity than Thermus aquaticus DNA
polymerase (determined using an adapted rpsL fidelity assay, Mo J.Y. et al., J. Mol. Biol. 1991; Fujii. S. et al., J. Mol. Biol. 1999). In
addition, owing to its enhanced processivity, VELOCITY exhibits not only high amplification rates up to 66 bp/s (equivalent to 15s/kb), but
also results in higher yields than most commercially available enzymes.