重组人表皮脂肪酸结合蛋白
货号：RD004-01
种属：本重组人表皮脂肪酸结合蛋白的氨基酸序列与人血清表皮脂肪酸结合蛋白的氨基酸序列完全相同。
规格：0.1mg (BCA法测定)
来源： 大肠杆菌
纯度： >90% (0SDS-PAGE测定).
产品形态：冻干粉（无菌过滤，0.5mg/ml，PBS缓冲液）
溶解：加0.2ml去离子水，室温下彻底溶解，最终浓度为0.5mg/ml。
保存条件： -20℃保存。建议溶解后分装保存，避免反复冻融。溶解后可以暂时保存在4℃至两周。
应用： ELISA、Western blotting
有效期：见包装。
质控检测：BCA-测定蛋白浓度
SDS PAGE-测定蛋白纯度
注意：本产品仅用于科研。

简介：
表皮脂肪酸结合蛋白（EFABP）属于细胞内脂肪酸结合蛋白（AFBP）家族，分子量15kDa。脂肪酸结合蛋白（AFBP）具有在腔内结合脂肪酸及相关复合物（胆汁酸或类维生素A）的能力。EFABP和AFABP紧密相关，都在脂肪细胞中表达。在肥胖和胰岛素抵抗两种模型中，EFABP的缺失可以提高系统的胰岛素敏感性。从EFABP缺失小鼠中分离出的脂肪细胞的胰岛素介导的葡萄糖转运能力也得到增强。相反地，脂肪组织高表达FABP的小鼠表现出系统的胰岛素敏感性降低。

Human Epidermal Fatty Acid Binding Protein (EFABP), Recombinant Protein
Cat. No.: BW004-01
Specificity: The amino acid sequence of recombinant human EFABP is 100% homologous to the amino acid sequence of human serum EFABP.
AA Sequence: MATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCIYE KVE (Total 133 amino acid residues, 15.2kDa)
广州百为生物技术有限公司
www.bio-way.com
Size: 0.1 mg (determined by BCA method)
Source: E. coli
Purity: >90% (SDS-PAGE analyzed).
Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer
Reconstitution: Add 0.2 ml deionized water and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Applications: ELISA, Western blotting
Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.

Quality Control Test: BCA - to determine content of the protein
SDS PAGE - to determine purity of the protein
Note: For research use only.

Introduction:
Human fatty acid binding protein EFABP is a 15 kDa member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds ( bile acids or retinoids) in an internal cavity. The fatty acid binding proteins AFABP and EFABP are closely related and both are expressed in adipocytes. Absence of EFABP resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Adipocytes isolated from EFABP-deficient mice also exhibited enhanced insulin-stimulated glucose transport capacity. In contrast, mice expressing high levels of EFABP in adipose tissue display reduced systemic insulin sensitivity