FITC标记的肌动蛋白相关蛋白2/3抗体
产品名称： FITC标记的肌动蛋白相关蛋白2/3抗体
英文名称： Anti-ARP2/FITC
产品编号： HZ-12524R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
- 联系人 : 鲍丽雯
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- 所在区域 : 上海
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Rabbit Anti-ARP2/FITC Conjugated antibody
FITC标记的肌动蛋白相关蛋白2/3抗体
| 英文名称 | Anti-ARP2/FITC |
| 中文名称 | FITC标记的肌动蛋白相关蛋白2/3抗体 |
| 别 名 | Actin like protein 2; Actin related protein 2; Actin-like protein 2; ACTIN-RELATED PROTEIN 2; ACTR2; ARP2 actin related protein 2 homolog (yeast); ARP2; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞粘附分子 细胞分化 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Pig, Cow, Xenopus laevis, Orangutan |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARP2/ARP3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia. Function: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Subunit: Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Subcellular Location: Cytoplasm; cytoskeleton. Cell projection. Similarity: Belongs to the actin family. ARP2 subfamily. Database links: Entrez Gene: 10097 Human Entrez Gene: 66713 Mouse Entrez Gene: 289820 Rat Entrez Gene: 396147 Chicken Omim: 604221 Human SwissProt: P53488 Chicken SwissProt: P61160 Human SwissProt: P61161 Mouse SwissProt: Q5M7U6 Rat Unigene: 643727 Human Unigene: 259045 Mouse Unigene: 102249 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
肌动蛋白聚合是多种细胞功能所必需的，包括趋化性、细胞迁移、细胞粘附和血小板活化。细胞通过从单体肌动蛋白中重新成核细丝、切断现有细丝以形成无钩的倒钩端或解开现有倒钩端来触发肌动蛋白聚合。肌动蛋白的成核是肌动蛋白聚合过程中的一个限制速率和不利的反应，因此需要Arp2/3复合物的参与，这有助于产生新的丝和促进肌动蛋白丝在分支网络中的端侧交联。ARP2／3复合物由肌动蛋白相关蛋白ARP2和ARP3和其他各种辅助蛋白组成。Arp2/3复合物通过结合肌动蛋白丝的尖端或通过与现有丝侧结合，促进肌动蛋白成核，并在带刺方向上成核生长。此外，Arp2 / 3复合物介导肌动蛋白细胞骨架的副产物，是由Rho家族小GTP酶的调节。针对GTP结合蛋白，Arp2 / 3复合物结合的Cdc42的基板，即WASP蛋白，并引发片状伪足和丝状伪足的形成。