HYBOND-N+-过滤耗材-耗材-生物在线
上海基星生物科技有限公司
HYBOND-N+

HYBOND-N+

商家询价

产品名称: HYBOND-N+

英文名称: HYBOND-N+

产品编号: RPN303B

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
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RPN119B HYBOND-N+, 50X11.9X7.8CM
RPN119N HYBOND-N, 50X11.9X7.8CM
RPN1210B HYBOND-N+,20X12CMX10CM
RPN1210N HYBOND-N, 20X12CMX10CM
RPN132B HYBOND-N+, 50X132MM DISCS
RPN132N HYBOND-N, 50X132MM DISCS
RPN132T HYBOND-NX NYLON MEMBRANE 50X1
RPN137B HYBOND-N+, 50X137MM DISCS
RPN137N HYBOND-N, 50X137MM DISCS
RPN137T HYBOND-NX NYLON MEMBRANE 50X1
RPN1510B HYBOND-N+, 20X15CMX10CM
RPN1510N HYBOND-N, 20X15CMX10CM
RPN1520B HYBOND-N+, 10X15CMX20CM
RPN1520N HYBOND-N, 20X15CMX20CM
RPN1520T HYBOND-NX NYLON MEMBRANE 10 X
RPN1732B HYBOND-N+, 50X132MM DISCS,GRID
RPN1737B HYBOND-N+, 50X137MM DISCS,GRID
RPN1782B HYBOND-N+, 50X82MM DISCS,GRIDD
RPN1787B HYBOND-N+, 50X87MM DISCS,GRIDD
RPN2020B HYBOND-N+, 10X20CMX20CM
RPN2020N HYBOND-N, 10X20CMX20CM
RPN2020T HYBOND-NX NYLON MEMBRANE 10 X
RPN203B HYBOND-N+, 20CMX3M
RPN203N HYBOND-N, 20CMX3M
RPN203T HYBOND-NX NYLON MEMBRANE 20CM
RPN2222B HYBOND-N+, 10X22CMX22CM
RPN2222N HYBOND-N, 10X22CMX22CM
RPN2222T HYBOND-NX NYLON MEMBRANE 10 X
RPN2250B HYBOND N+ 50 X 22.2 X 22.2 CM
RPN303B HYBOND-N+, 30CMX3M
RPN303N HYBOND-N, 30CMX3M
RPN303T HYBOND-NX NYLON MEMBRANE 30CM
RPN3050B HYBOND-N+, 30CMX50CM
RPN82B HYBOND-N+, 50X82MM DISCS
RPN82N HYBOND-N, 50X82MM DISCS
RPN82T HYBOND-NX NYLON MEMBRANE 50X8
RPN87B HYBOND-N+, 50X87MM DISCS
RPN87N HYBOND-N, 50X87MM DISCS

Amersham Hybond™-N+

  • Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm2¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
  • Recommended for all high density membrane arrays.

Amersham Hybond™-N+

Technical Information


Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.

 

  • Multiple reprobing is possible without loss of membrane integrity.
     
  • QC tested in genomic Southern blots using GE Healthcare products.
     
  • Special sizes available on request.

 

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Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C. bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.

 

Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.

 

TECHNICAL SPECIFICATIONS
Pore size  0.45 µm 
Nucleic acid size required  > 50 bp 

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=46939