Biotin-16-dCTP生物素标记物-生物分子-试剂-生物在线
武汉博欧特生物科技有限公司
Biotin-16-dCTP生物素标记物

Biotin-16-dCTP生物素标记物

商家询价

产品名称： Biotin-16-dCTP生物素标记物

英文名称： Biotin-16-dCTP

产品编号： orb64049

产品价格： 6539元

产品产地： UK

品牌商标： Biorbyt

更新时间： 2024-06-06T16:04:23

使用范围： null

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Catalog Number orb64049
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Description Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.
Concentration 1.0 mM-1.1 mM
Form/Appearance filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Purity ≥ 95% (HPLC)
MW Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
SMILES C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2
Formula C32H51N8O17P3S
Storage store at -20 °C
Note For research use only
Application notes Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase.
Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
Expiration Date 12 months from date of receipt.