p220.2载体表达EBNA1基因
产品名称: p220.2载体表达EBNA1基因
英文名称: p220.2载体表达EBNA1基因
产品编号: BiovectreP2202
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
Biovector Co.,LTD
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Order ID |
Name |
Description |
BiovectreP2202 |
p220.2 |
DNA.15uL,AmpR.Storage:-20℃ |
p220.2载体表达EBNA1基因,Epstein-Barr oriP, HSV-TK启动子+hyg潮霉素抗性基因,Amp原核抗性。
Transformation of plasmid DNA to competent E. Coli cells
- Thaw competent cells on ice. 20–200µL per tube
- Add max. 1-3µL of plasmid
- Mix very gently!
- Incubate the tubes on ice for 30 min
- Heat shock the cells for 45 sec to 2 min at 42°C
- Place the tubes immediately on ice for at least 2 min
- Add 800µL of SOC medium to each tube
- Incubate for 1 hour at 37°C and shake vigorously
- Spin down briefly and remove most supernatants
- Resuspend cell pellet with the rest SOC medium in the tube by pipetting