p220.2载体表达EBNA1基因-载体及构建-试剂-生物在线
Biovector Co.,LTD
p220.2载体表达EBNA1基因

p220.2载体表达EBNA1基因

商家询价

产品名称: p220.2载体表达EBNA1基因

英文名称: p220.2载体表达EBNA1基因

产品编号: BiovectreP2202

产品价格: 0

产品产地: Biovector Inc. USA

品牌商标: Biovector, Addgene, ATCC, Invi

更新时间: null

使用范围: null

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Product Information

Order ID

Name

Description

BiovectreP2202

p220.2

DNA.15uL,AmpR.Storage:-20

p220.2载体表达EBNA1基因,Epstein-Barr oriP, HSV-TK启动子+hyg潮霉素抗性基因,Amp原核抗性。

Transformation of plasmid DNA to competent E. Coli cells

    1. Thaw competent cells on ice. 20–200µL per tube
    2. Add max. 1-3µL of plasmid
    3. Mix very gently!
    4. Incubate the tubes on ice for 30 min
    5. Heat shock the cells for 45 sec to 2 min at 42°C
    6. Place the tubes immediately on ice for at least 2 min
    7. Add 800µL of SOC medium to each tube
    8. Incubate for 1 hour at 37°C and shake vigorously
    9. Spin down briefly and remove most supernatants
    10. Resuspend cell pellet with the rest SOC medium in the tube by pipetting