PEZZ 18 EXPRESION VECTOR-蛋白修饰-试剂-生物在线
上海基星生物科技有限公司
PEZZ 18 EXPRESION VECTOR

PEZZ 18 EXPRESION VECTOR

商家询价

产品名称： PEZZ 18 EXPRESION VECTOR

英文名称： PEZZ 18 EXPRESION VECTOR

产品编号： 27-4810-01

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
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            27-4810-01
            PEZZ 18 EXPRESION VECTOR
        

        

            28-4013-51
            HIS GRAVITRAP KIT
        

        

            28-4055-00
            GST MULTITRAP 4B
        

        

            28-4055-01
            GST MULTITRAP FF
        

        

            28-9523-59
            GST SpinTrap
        

        

            28-9523-60
            GST GraviTrap
        

        

            28-9523-61
            GST Buffer Kit
        

        

            28-9545-49
            PGEX-4T-1
        

        

            28-9545-50
            PGEX-4T-2
        

        

            28-9545-52
            PGEX-4T-3
        

        

            28-9545-53
            PGEX-5X-1
        

        

            28-9545-54
            PGEX-5X-2
        

        

            28-9545-55
            PGEX-5X-3
        

        

            28-9546-46
            PGEX-2TK
        

        

            28-9546-48
            PGEX-6P-1 VECTOR
        

        

            28-9546-50
            PGEX-6P-2 VECTOR
        

        

            28-9546-51
            PGEX-6P-3 VECTOR
        

        

            28-9546-53
            PGEX-2T 25 UG WI/DI
        

        

            28-9546-54
            PGEX-3X 25 UG WI/DI
        

        

            28-9546-56
            PGEX-1LT ECOR I/BAP
        

        

            11-0033-99
            HIS GRAVITRAP
        

        

            11-0034-00
            HIS BUFFER KIT
        

        

            27-4710-01
            ANTI-HIS ANTIBODY
        

        

            28-4009-89
            HIS MULTITRAP HP
        

        

            28-4009-90
            HIS MULTITRAP FF
        

        

            28-4013-53
            HIS SPINTRAP
        

        

            28-4039-43
            COLLECTION PLATE
        

        

            28-9321-71
            His SpinTrap Kit
        

        

            27-1583-01
            LIGHT PRIMER MIX
        

    







pEZZ 18 Protein A Gene Fusion Vector


pEZZ 18 Protein A Gene Fusion Vector


    
    
  • For rapid expression of secreted fusion proteins and their one-step purification using IgG Sepharose 6 Fast Flow
    • 



 


The phagemid pEZZ 18 contains the protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the protein A signal sequence. They are easily purified using IgG Sepharose 6 Fast Flow to which the “ZZ” domain binds tightly. Because of its folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.


Expression


Expression is controlled by the lacUV5 and protein A promoters and is not inducible. Elements of the protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert


Sequencing


M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector


Cloning


Inserts containing a stop codon will yield white colonies when grown on media containing X-gal


Host(s)


E. coli strains carrying a lac deletion but capable of 

a-complementation of lacZ’.




pEZZ 18 Protein A Gene Fusion Vector — Technical Specifications





    
        

            Complete Packsize
            25 µg
        

        

            Order Information
            For research use only. Not for diagnostic or therapeutic purposes.
        

        

            Selectable Marker [s]
            Plasmid confers resistance to 70 μg/ml ampicillin
        

    
    




    
        

            
            
Licensing

            		
            
            
For licensing information, see the Licensing Statements page in the About Us section