大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒
产品名称： 大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒
英文名称： FⅧ-Ag
产品编号： DMD0732
产品价格： 0
产品产地： 上海
品牌商标： DM
更新时间： null
使用范围： null
上海笃玛生物科技有限公司
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上海笃玛生物科技有限公司长期长期供应原装ELISA试剂盒，公司与国内知名酶联免疫分析试剂盒（ELISA试剂盒）厂家成为战略合作伙伴。各种属ELISA试剂盒产品种类齐全，质量可靠。
产品名称：大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒
英文名称：Rat coagulation factor VIII related antigen (F VIII -Ag) ELISA kit.
贮藏条件：2-8℃低温保存
有效期：6个月
特异性: 可同时检测天然或重组的，且与其他相关蛋白无交叉反应。
检测种属：人、大小鼠、兔、羊、猴、猪、豚鼠ELISA检测试剂盒等种属。
适用范围：此试剂盒仅用于科研实验，禁用于临床
大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒笃玛生物试验原理
试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将生物素标记的抗体同时温育。洗涤后，加入亲和素标记过的HRP。再经过温育和洗涤，去除未结合的酶结合物，然后加入底物A、B，和酶结合物同时作用。产生颜色。颜色的深浅和样品中的浓度呈比例关系。
大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒操作步骤：
1、加样：分别设空白孔(空白对照孔不加样品及酶标试剂，其余各步操作相同)、标准孔、待测样品孔。在酶标包被板上标准品准确加样50μl，待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
2、温育：用封板膜封板后置 37℃温育30分钟。
3、配液：将30倍浓缩洗涤液用蒸馏水30倍稀释后备用
4、洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此
重复5次，拍干。
5、加酶：每孔加入酶标试剂50μl，空白孔除外。
6、显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色
10 分钟.
7、终止：每孔加终止液50μl，终止反应(此时蓝色立转黄色)。
8、测定：以空白空调零，450nm波长依序测量各孔的吸光度(OD值)。测定应在加终止液后15分钟以内进行。
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