Membrane Protein Purification Kit

• Simplify detergent screening of histidine-tagged membrane proteins.
• Simultaneous evaluation of solubilization and purification efficiency.
• Rapid and easy screening in the convenient magnetic bead format.
• Ready-to-use detergent solutions.
• Easy-to-follow protocol.

Technical Information

Membrane Protein Purification Kit for histidine-tagged membrane proteins allows rapid and easy detergent screening for simultaneous evaluation of solubilization and purification efficiency.

Membrane Protein Purification Kit is designed for efficient detergent screening of histidine-tagged membrane proteins. Small aliquots of cell membranes are solubilized in different detergents followed by rapid purification using His Mag Sepharose™ Ni. The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration, or light scattering. Membrane Protein Purification Kit is sufficient for two complete screening experiments according to the included protocol.

The magnetic bead format is excellent for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices, while the visibility of the beads ensures reliable collection of the bound histidine-tagged membrane proteins in the screening and purification procedure. Purification of target membrane proteins for a number of analytical and/or other downstream applications can be achieved within a few hours. For convenient purification of larger amounts of membrane proteins, use prepacked HisTrap™ HP columns.

^*Analytical grade, >99% by HPLC analysis; all detergents are from Anatrace™ Maumee, Ohio, USA (Affymetrix).
^†Approximate critical micelle concentrations (CMC) in water at 20°C.