上海复蒙基因生物科技有限公司是美国Sciencell公司在中国正规一级代理：

         美国ScienCell研究实验室（ www.sciencellonline.com ）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
        

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。 确保了实验结果的重复性和连贯性。

请登录Sciencell公司官方网站（ www.sciencellonline.com 或 www.sciencell-sh.com ）以确保购买正规公司产品。

产品：人IGFBP-3酶联免疫试剂盒

货号：EK0386

Description

Insulin-like growth factor-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 expression. IGFBP-3, well-characterized as the carrier of IGF, has been reported to have intrinsic bioactivity that is independent of IGF binding; IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated, but not in terminally differentiated, chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extra cellular matrix. IGFBP-3 is located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

ScienCells human IGFBP-3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IGFBP-3 specific-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Bioyin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IGFBP-3 captured in strips .

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice.

Reference

1. Deal, C.; Ma, J.; Wilkin, F.; Paquette, J.; Rozen, F.; Ge, B.; Hudson, T.; Stampfer, M.; Pollak, M. Novel promoter polymorphism in insulin-like growth factor-binding protein-3: correlation with serum levels and interaction with known regulators. J. Clin. Endocr. Metab. 86: 1274-1280, 2001.
2. Spagnoli, A.; Torello, M.; Nagalla, S. R.; Horton, W. A.; Pattee, P.; Hwa, V.; Chiarelli, F.; Roberts, C. T., Jr.; Rosenfeld, R. G. Identification of STAT-1 as a molecular target of IGFBP-3 in the process of chondrogenesis. J. Biol. Chem. 277: 18860-18867, 2002.
3. Weinzimer, S. A.; Gibson, T. B.; Collett-Solberg, P. F.; Khare, A.; Liu, B.; Cohen, P. Transferrin is an insulin-like growth factor-binding protein-3 binding protein. J. Clin. Endocr. Metab. 86: 1806-1813, 2001.
4. Ehrenborg, E.; Larsson, C.; Stern, I.; Janson, M.; Powell, D. R.; Luthman, H. Contiguous localization of the genes encoding human insulin-like growth factor-binding proteins 1 (IGBP1) and 3 (IGBP3) on chromosome 7. Genomics 12: 497-502, 1992.
 

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Sciencell公司其它产品：
 
原代细胞（纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离。）
原代细胞专用培养基
无血清培养
干细胞无血清培养基（骨髓间充质干细胞、胚胎干细胞）
原代细胞转染试剂盒等。

        复蒙基因|FMGBio(www.fmgbio.com/   www.sciencell-sh.com/   )成立于2005年， 是专业从事牛血清生产、销售高端的原代细胞类科研产品。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本，而且将极大地提高人类的健康质量。

        复蒙基因|FMGBio现有的产品：美国Sciencell（原代细胞、原代细胞专用培养基、无血清培养基）、3D Bioteck（三维细胞培养支架）、年产1000万毫升的内蒙古牛血清生产基地等。

       复蒙基因|FMGBio的PI团队已经发展到专职PI 20人，联席PI 312人，其中大多数拥有海外背景。截止到2009年3月，以PI或联席PI为第一作者发表SCI 论文1682 篇（总IF 值为5721.82，其中影响因子IF>5.0 的论文216 篇，IF>10.0论文32 篇）。

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