蛋白酶 K 溶液（20 mg/ml）

附带条件

特技

Proteinase K是一种在脂肪族、芳香族或疏水氨基酸的羧基侧裂解肽键的内溶蛋白酶。蛋白酶K被分类为丝氨酸蛋白酶。被这种酶水解的最小肽是四肽。

特技

准备好的溶液在很宽的反应条件下活性

船首

小鼠尾细胞基因组DNA的分离、脱除DNase和RNase的脱氧核糖核酸和核糖核酸酶在分离组织和细胞株DNA和RNA时的酶定位测定提高PCR产物的克隆效率

特赦

一个单位的酶释放福林阳性氨基酸和肽对应于1μmol酪氨酸在1分钟在37°C以变性血红蛋白为底物。

附带条件

源Pichia pastoris细胞编码的基因编码三角蕊花内切酶蛋白酶（Proteinase K）。

质量控制：缺乏适当的质量试验证实的内切、外脱氧核糖核酸酶和核糖核酸酶。

概述

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease ). The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

特点

• Ready-to-use solution Active in a wide range of reaction conditions

应用

Isolation of genomic DNA from mouse tail Isolation of genomic DNA from cultured cells Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines Determination of enzyme localization Improving cloning efficiency of PCR products

组份

One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine in 1 min at 37°C using denatured hemoglobin as substrate.

技术规格