Overview

Product name
cAMP Direct Immunoassay Kit


Product overview
The cAMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes the recombinant Protein G coated 96-well plate to anchor cAMP polyclonal antibody to the plate. cAMP-HRP conjugate directly competes with cAMP from the sample to bind to the cAMP specific antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in your samples. The kit provides a new acetylation procedure that improves the detection signal significantly. The kit can detect ~0.1-10 pmol/50 µl cAMP sample.


Notes
The component in reagent Vial A and B is sensitive to evaporation. The vials should be tightly capped always and has to be kept only at 4C. Should not be left at room temperature. 

 


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C


Storage buffer
Preservative: None

 



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
10X cAMP Assay Buffer
            
WM
            
1 x 25ml
        
        

            
Acetylating Reagent A
            
Violet
            
1 x 750µl
        
        

            
Acetylating Reagent B
            
Brown
            
1 x 1.5ml
        
        

            
cAMP-HRP
            
Green
            
1 vial
        
        

            
HRP Developer
            
Amber
            
1 x 10ml
        
        

            
Neutralizing Buffer
            
NM
            
1 x 7.5ml
        
        

            
Rabbit Anti-cAMP polyclonal Antibody
            
Red
            
1 vial
        
        

            
Standard cAMP (10 nmol)
            
Yellow
            
1 x 10µl
        
    


http://www.abcam.com/cAMP-Direct-Immunoassay-Kit-ab65355.html